Methodological Advances in Auxin and Cytokinin Biology -- Analytical Determination of Auxins and Cytokinins -- Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction -- Microbial Manipulation of Auxin and Cytokinins in Plants -- A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling -- Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity -- Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK) -- Quantification of Cytokinin Levels and Responses in Abiotic Stresses -- Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidisInfection in Arabidopsis thaliana -- Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies -- Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface -- Analyzing Cytokinin Responses during Plant-Nematode Interactions -- Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin -- A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks -- Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots -- High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation. .

This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.