Rapid Detection of Adenovirus from Fecal Specimens -- Alphaviruses -- Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR -- Enteroviruses and Rhinoviruses -- Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction -- Hepatitis Viruses -- Herpesviruses -- Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper -- PCR for the Detection of Influenza Viruses in Clinical Material -- Lyssaviruses -- Methods for Detecting Antimeasles, Mumps, and Rubella Virus Antibodies -- Papillomaviruses -- Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization -- Polioviruses -- Poxviruses -- Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections -- Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis -- Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNA by Calorimetric Enzymatic Immunoassay -- Time-Resolved Fluorescence -- Enzyme-Linked Immunosorbent Assays -- Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology -- Robotic Analyzers -- The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction -- Recombinant Antigens in Viral Diagnosis -- NASBA -- Oligonucleotides.

In Diagnostic Virology Protocols, internationally recognized experts present cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory using only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. The book also includes a number of chapters concisely evaluating many emerging technologies and their associated instrumentation. Diagnostic Virology Protocols inaugurates a new and exciting era in which a wide variety of novel technologies are combining to enable both researchers and clinicians alike to perform rapid, accurate, sensitive, and robust analyses of viral diseases. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects. All those in experimental and clinical virology today will find Diagnostic Virology Protocols the new standard resource for methodology, and wholly indispensable in their day-to-day work. .