Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior -- Baculovirus Molecular Biology/Development of Recombinant Baculoviruses -- to Baculovirus Molecular Biology -- Baculovirus Transfer Vectors -- Recombinant Baculovirus Isolation -- Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System -- Insect Cell Culture -- Available Lepidopteran Insect Cell Lines -- Lepidopteran Insect Cell Line Isolation from Insect Tissue -- Development of Serum-Free Media for Lepidopteran Insect Cell Lines -- Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses -- Protein Production with Recombinant Baculoviruses -- Small-Scale Protein Production With the Baculovirus Expression Vector System -- Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System -- Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors -- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -- Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System -- Recombinant Protein Production with Transformed Insect Cells -- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -- Transformation of Drosophila Cell Lines -- Transforming Lepidopteran Insect Cells for Improved Protein Processing -- Baculovirus Development and Production for Use as Insecticides -- to the Use of Baculoviruses as Biological Insecticides -- Baculovirus Insecticide Production in Insect Larvae -- Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses -- Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System -- Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy -- Alternative Bioreactor Strategy for Probing Infection and Production -- Methods for Gene Silencing With RNAi -- Using the Baculovirus/Insect Cell System to Study Apoptosis -- Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells.

Baculovirus Expression Protocols is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Protocols provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.