Preparation and Titration of CsCl-Banded Adenovirus Stock -- Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units -- Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants -- Manipulation of Early Region 4 -- Adenovirus DNA Packaging -- Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome -- Construction of Mouse Adenovirus Type 1 Mutants -- Generation of Adenovirus-Specific Cytotoxic T Lymphocytes -- Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells -- Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells -- Role of Natural Killer (NK) Cells in Immunity to Adenoviruses -- Determination of the Transforming Activities of Adenovirus Oncogenes -- Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members -- In Vitro Transcription -- Cell Microinjection -- Immunoprecipitation of E1 A-Containing Protein Complexes -- Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-lnfected Cells -- Adenovirus Entry into Cells -- Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining -- Characterization of the Adenovirus Fiber Protein -- Large-Scale Purification and Crystallization of Adenovirus Hexon -- Adenovirus Protease -- Methods for Growth and Purification of Enteric Adenovirus Type 40 -- Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA -- Phylogenetic Analysis of Adenovirus Sequences.

In Adenovirus Methods and Protocols, William S.M. Wold has organized a collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion, as well as tried and true step-by-step instructions. Adenovirus Methods and Protocols will be useful to both entry-level and senior scientists seeking to enter the adenovirus field, to researchers from other areas wishing to construct adenovirus vectors for their own research, and to adenovirologists wanting to enter new sectors of research. Its cutting-edge techniques are certain to make it today's reference of choice, one from which even expert researchers will learn many productive and time-saving techniques.