The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae -- The Pathogenesis of Disease Due to Type b Haemophilus influenzae -- General Methods for Culturing Haemophilus influenzae -- Transformation of Haemophilus influenzae -- Diagnosis of Infection -- Characterization of Plasmids -- Protein Characterization by Two-Dimensional Gel Electrophoresis -- Monitoring Gene Expression Using DNA Arrays -- Gene Expression Technology -- The Genome Sequence of Haemophilus influenzae -- Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae -- Mutagenesis of H. influenzae -- Transposon Tn 10 -- In Vivo Expression of Bacterial Genes During Human Infections -- ELISA -- Opsonophagocytosis Assay Using Flow-Cytometry -- In Vitro Models of Infection I-Human Respiratory Tissue Organ Culture -- In Vitro Models of Infection II-Human Umbilical Vein Endothelial Cells (HUVECs) System -- Animal Models.

As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models. Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.