to Supercritical Fluids and Their Applications -- Supercritical Fluid Extraction of Caffeine from Instant Coffee -- Supercritical Fluid Extraction of Nitrosamines from Cured Meats -- Supercritical Fluid Extraction of Melengestrol Acetate from Bovine Fat Tissue -- Supercritical Fluid Extraction of Polychlorinated Biphenyls from Fish Tissue -- Isolation of Polynuclear Aromatic Hydrocarbons from Fish Products by Supercritical Fluid Extraction -- Supercritical Fluid Extraction of Mycotoxins from Feeds -- Supercritical Fluid Extraction of Pigments from Seeds of Eschscholtzia californica Cham -- Supercritical Fluid Extraction of Flumetralin from Tobacco Samples -- Supercritical Fluid Extraction and High Performance Liquid Chromatography Determination of Carbendazim in Bee Larvae -- Supercritical Fluid Extraction Coupled with Enzyme Immunoassay Analysis of Soil Herbicides -- The Supercritical Fluid Extraction of Drugs of Abuse from Human Hair -- Application of Direct Aqueous Supercritical Fluid Extraction for the Dynamic Recovery of Testosterone Liberated from the Enzymatic Hydrolysis of Testosterone-?-D-Glucuronide -- Analysis of Anabolic Drugs by Direct Aqueous Supercritical Fluid Extraction Coupled On-Line with High-Performance Liquid Chromatography -- Detection of Beta-Blockers in Urine and Serum by Solid-Phase Extraction-Supercritical Fluid Extraction and Gas Chromatography-Mass Spectrometry -- On-Line SFE-SFC for the Analysis of Fat-Soluble Vitamins and Other Lipids from Water Matrices -- Determination of Artemisinin in Artemisia annua L. by Off-Line Supercritical Fluid Extraction and Supercritical Fluid Chromatography Coupled to an Evaporative Light-Scattering Detector -- Analysis of Cannabis by Supercritical Fluid Chromatography with Ultraviolet Detection -- Direct Chiral Resolution of Optical Isomers of Diltiazem Hydrochloride by Packed Column Supercritical Fluid Chromatography -- Determination of Salbutamol Sulfate and Its Impurities in Pharmaceuticals by Supercritical Fluid Chromatography -- Packed Column Supercritical Fluid Chromatographic Determination of Acetaminophen, Propyphenazone, and Caffeine in Pharmaceutical Dosage Forms -- Analysis of Shark Liver Oil by Thin-Layer and Supercritical Fluid Chromatography -- Enzymatically Catalyzed Transesterifications in Supercritical Carbon Dioxide -- Transesterification Reactions Catalyzed by Subtilisin Carlsberg Suspended in Supercritical Carbon Dioxide and in Supercritical Ethane -- Enzymatic Synthesis of Peptide in Water-Miscible Organic Solvent/Supercritical Carbon Dioxide -- Micronization of a Polysaccharide by a Supercritical Antisolvent Technique -- Rapid Expansion of Supercritical Solutions Technology -- Supercritical Fluid Aerosolized Vitamin E Supplementation -- Extraction of Biologically Active Substances from Wood -- The Deposition of a Biocide in Wood-Based Material -- Critical Point Drying of Biological Specimens for Scanning Electron Microscopy -- Staining of Fingerprints on Checks and Banknotes Using Ninhydrin.

Because of their unique properties and relatively low environmental impact, supercritical fluids have proven highly useful in the extraction and separation of organic compounds, in particle production, as reaction media, and for the destruction of toxic waste. In Supercritical Fluid Methods and Protocols, experienced practitioners present detailed accounts of a wide variety of techniques using supercritical fluids. These range from the supercritical fluid extraction methods for numerous compounds to the ninhydrin staining of fingerprints on checks and banknotes, and from the detection of impurities in pharmaceuticals to a wide variety of applications throughout environmental and food science, and across analytical, clinical, and medicinal chemistry. Detailed step-by-step instructions enable users to apply these essential techniques successfully the first time, and include modifications that permit their effective adaptation to novel experimental or process conditions. For each application, additional discussions provide needed background information, lists of materials and apparatus, and advice about common pitfalls and how to avoid them. Versatile and comprehensive, Supercritical Methods and Protocols offers both novice and experienced investigators and laboratory analysts powerful tools that will enable successful biological and bioprocess analyses and optimizations today.