to DNA Sequencing -- The Universal Primers and the Shotgun DNA Sequencing Method -- M13 Sequencing -- Primer Design and Primer-Directed Sequencing -- Direct Sequencing of DNA Produced in a Polymerase Chain Reaction -- Solid Phase Fluorescent Sequencing of the CFTR Gene -- Shotgun DNA Sequencing -- Cycle Sequencing -- Cycle Sequencing of Polymerase Chain Reaction-Amplified Genomic DNA with Dye-Labeled Universal Primers -- Automated Fluorescent DNA Sequencing on the ABI PRISM 377 -- Automated Fluorescent DNA Sequencing on the ABI PRISM 310 Genetic Analyzer -- Fluorescent Sequencing Protocols for the ALF -- Fluorescent Sequencing for Heterozygote Mutation Detection -- Sequence Databases and the Internet -- DNA Sequencing by Capillary Array Electrophoresis.

Since the publication of the first edition of DNA Sequencing Protocols in 1993, major advances in its technology have made PCR-based semiautomated fluorescent sequencing the norm and enabled the complete sequencing of complex genomes. In DNA Sequencing Protocols, 2nd Edn., Colin Graham and a team of leading investigators and expert clinical scientists update the first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. Each method provides detailed, step-by-step instructions to ensure successful results, and includes helpful notes identifying and addressing potential problems. This second edition offers new material on automated DNA sequencers, capillary DNA sequencers, heterozygote mutation detection, web-based sequencing databases and genome sequencing sites, and the human genome project. State-of-the-art and highly practical, DNA Sequencing Protocols, 2nd Edn. constitutes an essential laboratory handbook for geneticists and molecular biologists, offering concise, easy-to-follow methods that will impact today's genome sequencing projects, improve the accuracy and quality of DNA sequences obtained by smaller laboratories, and help lay the foundation for molecular diagnostics.