Liposomes Came First: The Early History of Liposomology -- Thin Film Hydration followed by Extrusion Method for Liposome Preparation -- Preparation of DRV Liposomes -- Method of Simultaneous Analysis of Liposome Components by HPTLC/FID -- Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale -- Liposome Formulations of Hydrophobic Drugs -- A "Dock and Lock" Approach to Preparation of Targeted Liposomes -- Coupling of Ligands to the Liposome Surface by Click Chemistry -- Elastic Liposomes for Topical and Transdermal Drug Delivery -- Determination of the Sub-Cellular Distribution of Liposomes using Confocal Microscopy -- Environmental Scanning Electron Microscope Imaging of Vesicle Systems -- Liposome Biodistribution via Europium Complexes -- Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy -- Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes -- Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes -- Long-Circulating, pH-Sensitive Liposomes -- Anionic pH-Sensitive Lipoplexes -- Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes -- Preparation and Physical Characterization of DNA Binding Cationic Liposomes -- Fluorescence Resonance Energy Transfer (FRET) Based Analysis of Lipoplexes -- Targeted Magnetic Liposomes Loaded with Doxorubicin -- Stable Discoidal Bicelles - A Platform of Lipid Nanocarriers for Cellular Delivery.

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Liposomes: Methods and Protocols, Second Edition is a valuable resource for graduate students, post-doctoral researchers, and established investigators utilizing lipid-based systems in the fields of cell and molecular biology, drug delivery, and physical chemistry. .