Protocols -- Preparation of Human Chromosomal Painting Probes From Somatic Cell Hybrids -- Fluorescent In Situ Hybridization Using Chromosome- Specific DNA Libraries -- Reverse Chromosome Painting -- FISH Detection on DAPI-Banded Chromosomes -- Generation of Alphoid DNA Probes for Fluorescence In Situ Hybridization (FISH) Using the Polymerase Chain Reaction -- In Situ Hybridization Using Synthetic Oligomers as Probes for Centromere and Telomere Repeats -- Mapping Human YAC Clones by Fluorescence In Situ Hybridization Using Alu-PCR from Single Yeast Colonies -- FISH of Alu-PCR-Amplified YAC Clones and Applications in Tumor Cytogenetics -- Primed In Situ (PRINS) Labeling of DNA -- Free Chromatin Mapping by FISH -- Use of DNA- Halo Preparations for High- Resolution DNA In Situ Hybridization -- In Situ Hybridization of Meiotic Prophase Chromosomes -- Strand-Specific Fluorescence In Situ Hybridization for Determining Orientation and Direction of DNA Sequences -- Radioactive In Situ Hybridization to Replication-Banded Chromosomes -- Gene Mapping Using 3H-Labeled Heterologous Probes -- Dual- label fluorescence in situ hybridization detection of individual chromosomes in sperm using ejaculated semen -- In situ hybridization to drosophila polytene chromosomes -- In situ hybridization to polytene chromosomes of drosophila melanogaster and other dipteran species -- Microsurgical isolation of native polytene chromosomes of drosophila melanogaster for in situ molecular observation -- PCR In situ hybridization -- Detection of Virus Nucleic Acids by Radioactive and Nonisotopic In Situ Hybridization -- Sensitive Immunocytochemical Detection of Viral Genomes -- Primed In Situ (PRINS) Labeling of RNA -- Hybridization Histochemistry Using Radiolabeled Oligodeoxyribonucleotide Probes -- mRNA In Situ Hybridization to In Vitro Cultured Cells -- Quantitative In Situ Hybridization Using Radioactive Probes in the Study of Gene Expression in Heterocellular Systems -- Tissue Distribution of Gene Expression in Mammalian Development -- Subcellular Location of mRNA by Electron Microscope Hybridization Histochemistry -- Reveiws -- Principle of Digital Imaging Microscopy -- Application of In Situ Hybridization for the Detection of Virus Nucleic Acids -- Positional Cloning and Multicolor In Situ Hybridization Principles and Protocols -- Characterization of Constitutive Marker Chromosomes in Humans -- Detection of Chromosomal Aberrations in Interphase and Metaphase Cells in Prenatal and Postnatal Studies.

In situ hybridization is now recognized as an important technique in many areas of molecular biological research and its associated cli- cal studies. There is very little doubt that the applications of this te- nique will multiply in coming years since older methods are rapidly superseded by newer ones that enable the investigation of even more sophisticated biological questions and clinical problems. For the n- ice, there should thus be a sense of urgency to acquire and extend the range of this technique in order not to miss out on the large body of information that can result from its application. During the explosive growth phase of most technical innovations, such knowledge is dif- cult to acquire systematically. The aim of In Situ Hybridization Pro- cols then is to bring together a wide range of detailed laboratory protocols covering different areas of the in situ hybridization technique in order to assist such workers in rapidly advancing toward this goal. With this aim in mind, each of the protocol chapters opens with a detailed Materials section, and an easy-to-follow Methods section. Many readers will find the section headed Notes particularly useful. This s- tion contains an abundance of information and troubleshooting tips that come straight from the horse's mouth, and that are not often given in the restricted methods sections of standard research publications. For this reason, the book should also appeal to experienced workers wi- ing to add new methods to their repertoires.