Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen -- Characterization of B-Cell Maturation in the Peripheral Immune System -- Identification of B-Cell Subsets -- Analysis of B-Cell Life-Span and Homeostasis -- Differentiation of B Lymphocytes From Hematopoietic Stem Cells -- Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System -- Conditional Gene Mutagenesis in B-Lineage Cells -- Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells -- T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences -- of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors -- Immunoglobulin Class Switching -- In Vitro and In Vivo Assays of B-Lymphocyte Migration -- Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo -- B-Cell Signal Transduction -- Isolation of Lipid Rafts From B Lymphocytes -- Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells -- Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice -- Analysis of B-Cell Signaling Using DT40 B-Cell Line -- B-Cell Development and Pre-B-1 Cell Plasticity In Vitro.

Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.