Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells -- Processing Biological Tissues for Ultrastructural Study -- Processing Plant Tissues for Ultrastructural Study -- Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy -- Ultramicrotomy for Biological Electron Microscopy -- Poststaining Grids for Transmission Electron Microscopy -- Negative Staining of Thinly Spread Biological Samples -- Recent Advances in High-Pressure Freezing -- Cryoultramicrotomy -- Cell-Free Extract Systems and the Cytoskeleton -- Electron Microscopy In Situ Hybridization -- Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections -- Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry -- Fluorescence-Integrated Transmission Electron Microscopy Images -- Quantitative Immunoelectron Microscopy -- Electron Crystallography of Membrane Proteins -- Cryoelectron Microscopy of Icosahedral Virus Particles -- Three-Dimensional Reconstruction of Chromosomes Using Electron Tomography -- Whole-Mount Immunoelectron Tomography of Chromosomes and Cells -- Three-Dimensional Cryotransmission Electron Microscopy of Cells and Organelles -- Electron Energy-Loss Spectroscopy as a Tool for Elemental Analysis in Biological Specimens -- Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens -- Variable Pressure and Environmental Scanning Electron Microscopy -- Cryoplaning Technique for Visualizing the Distribution of Water in Woody Tissues by Cryoscanning Electron Microscopy -- X-Ray Microanalysis in the Scanning Electron Microscope -- Botanical X-Ray Microanalysis in Cryoscanning Electron Microscopy -- Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research -- Using SIMS and MIMS in Biological Materials.

In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.