Electrophoretic Techniques -- Field-Inversion Gel Electrophoresis -- Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF) -- Separation and Size Determination of DNA over a 10-,200 kbp Range -- PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE) -- Transverse Alternating-Field Electrophoresis -- Rotating Field Gel Electrophoresis (ROFE) -- Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE) -- Auxiliary Methods -- Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis -- Encapsulation of Cells in Agarose Beads -- Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE) -- Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis -- Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases -- Desktop Digital Imaging -- Biological Applications -- Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE) -- Protozoan Genomes -- Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis -- Analysis of Yeast Artificial Chromosome Clones -- Strategies for Mapping Large Regions of Mammalian Genomes -- Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA -- Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly Repeated DNA -- Construction of Lambda Libraries from Large PFGE Fragments -- In-Gel Detection of DNA -- Theories and Observations -- Theories of Pulsed-Field Gel Electrophoresis -- The Dynamics of DNA Gel Electrophoresis -- Orientation of the Agarose Matrix by Pulsed Electric Fields -- Reptation Theory of Pulsed Electrophoresis and Trapping Electrophoresis -- Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).