Computational Modeling of the Skin Barrier -- In Vitro Human Skin Segmentation and Drug Concentration-Skin Depth Profiles -- Transcriptional Regulation of Epidermal Barrier Formation -- Epidermal Permeability Barrier  (EPB) Measurement in Mammalian Skin -- Assessment of Permeability Barriers to Macromolecules in the Rodent Endometrium at the Onset of Implantation -- Assessment of Intestinal Permeability in (Premature) Neonates by Sugar Absorption Tests -- Analysis of Epithelial Cell Shedding and Gaps in the Intestinal Epithelium -- Studying Permeability in a Commonly Used Epithelial Cell Line: T84 Intestinal Epithelial Cells -- Optimization of the Caco-2 Permeability Assay to Screen Drug Compounds for Intestinal Absorption and Efflux -- Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion -- Monitoring of the Dynamics of Epithelial Dome Formation Using a Novel Culture Chamber for Long-Term Continuous Live-Cell Imaging -- Measuring Permeability in Human Retinal Epithelial Cells (ARPE-19): Implications for the Study of Diabetic Retinopathy -- Analysis of Epithelial Barrier Integrity in Polarized Lung Epithelial Cells -- Permeability of Differentiated Human Urothelium In Vitro -- Phenotyping the Claudin 11 Deficiency in Testis: From Histology to Immunohistochemistry -- An In Vitro System to Study Sertoli Cell Blood-Testis Barrier Dynamics -- Analysis of Endothelial Barrier Function In Vitro -- Role of Endothelial Cell-Cell Junctions in Endothelial Permeability -- In Vitro Analyses of Endothelial Cell Permeability -- Mechano-Transduction and Barrier Regulation in Lung Microvascular Endothelial Cells -- Role of Caveolin-1 in the Regulation of Pulmonary Endothelial Permeability -- Assessment of Endothelial Permeability and Leukocyte Transmigration in Human Endothelial Cell Monolayers -- Permeability of Endothelial Barrier: Cell Culture and In Vivo Models -- Size-Selective and In Vitro Assessment of Inner Blood Retina Barrier Permeability -- Assessment of Permeability in Barrier Type of Endothelium in Brain Using Tracers: Evans Blue, Sodium Fluorescein, and Horseradish Peroxidase -- In Vitro and In Vivo Methods for Assessing FcRn-Mediated Reverse Transcytosis Across the Blood-Brain Barrier -- Evaluation of VEGF-Induced Vascular Permeability in Mice -- In Vivo Measurement of Glioma-Induced Vascular Permeability -- In Vivo Optical Imaging of Ischemic Blood Brain Barrier Disruption.

The significant biological subject, the permeability barrier, is incredibly diverse and vital for a vast assortment of crucial functions in the body.  In Permeability Barrier: Methods and Protocols, a variety of experienced researchers contribute techniques to study this complex system in its many forms.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Permeability Barrier: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.