In Vivo Models of Inflammatory Bowel Disease and Colitis-Associated Cancer -- Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer -- Utility of Endoscopic Ultrasound-Guided Fine-Needle Aspiration for Preclinical Evaluation of Therapies in Cancer -- Modelling Intestinal Carcinogenesis Using In Vitro OrganoidCultures -- Protocols to Evaluate Cigarette Smoke-induced Lung Inflammationand Pathology in Mice -- Tracking Competent Host Defence to Chronic Inflammation: An In vivo Model of Peritonitis -- Citrobacter rodentium Infection Model for the Analysis of Bacterial Pathogenesis and Mucosal Immunology -- In Vivo Infection Model of Severe Influenza a Virus -- In Vivo Models for Inflammatory Arthritis -- Isolation of Mouse Primary Gastric Epithelial Cells to Investigate the Mechanisms of Helicobacter pylori Associated Disease -- Dissecting lnterleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer -- Laser Microdissection of Cellular Compartments for Expression Analyses in Cancer Models -- Tissue Processing for Stereological Analyses of Lung Structure in Chronic Obstructive Pulmonary Disease -- Quantifying Caspase-1 Activity in Murine Macrophages -- Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation -- Analysis of DNA Methylation in Tissues Exposed to Inflammation -- A Comprehensive Protocol Resource for Performing Pooled shRNA and CRISPR Screens -- Immuno-detection of Immature and Bioactive Forms of the Inflammatory Cytokine IL-18 -- Optimisation Techniques for miRNA Expression in Low Frequency Immune Cell Populations -- Assessing the cGAS-cGAMP-STING Activity of Cancer Cells -- Expression and Purification of JAK1 and SOCS1 for Structural and Biochemical Studies -- Production of Recombinant Killer Immunoglobulin-like Receptors for Crystallography and Luminex Based Assays -- Isolation and Characterisation of Mouse Intrahepatic Lymphocytes by Flow Cytometry.

This volume provides a broad spectrum of research models, techniques, and protocols. Chapters are divided into sections providing detailed information on the background and context for the chosen topic of interest, a list of the necessary materials and reagents needed for each topic, as well as the step-by-step laboratory protocols and methodologies for the successful and reproducible execution of each topic. In addition, each chapter includes notes to provide tips avoiding known pitfalls, troubleshooting advice and elaborate further on specific materials or methods. Authoritative and practical, Inflammation and Cancer: Methods and Protocols aims to provide information to students and researchers at all levels of experience for further study into this vital field.