Identification and Analysis of Precursors to Invasive Pancreatic Cancer -- Tissue Microarrays: Construction and Use -- Immunohistochemistry of Pancreatic Neoplasia -- A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer -- Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications -- Considerations for Sequencing Analyses of Pancreatic Cancer Progression and Metastasis -- DNA Methylation Analysis in Human Cancer -- Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer Patients -- Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer Vaccine -- The Prevention and Genetics of Pancreatic Cancer - A Programmatic Approach -- Development of Orthotopic Pancreatic Tumor Mouse Models -- Evaluating Dietary Compounds in Pancreatic Cancer Modeling Systems -- Quantification of Murine Pancreatic Tumors by High Resolution Ultrasound -- Microdissection and Culture of Murine Pancreatic Ductal Epithelial Cells -- Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem Cells -- Analysis of Transplanted Bone Marrow Derived Cells in Chronic Pancreatitis -- Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In Vivo -- The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer Cells -- Analysis of Tumor Associated Mucin Glycotopes by Western Transfer Methods -- Evaluating Tumor Angiogenesis -- Pooled Genome-wide RNAi Screenings -- Pooled ShRNA screenings: Computational Analysis.  .

Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA.  Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes.  The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer.   It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer. .