The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications -- Two-Photon Excitation Microscopy And Its Applications In Neuroscience -- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy -- Fluorescence Microscopy Based RNA Interference Screening -- Fluorescence Resonance Energy Transfer Microscopy (FRET) -- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy -- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation -- Fluorescence Correlation Spectroscopy -- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules -- Practical Structured-Illumination Microscopy -- 4Pi Microscopy of the Nuclear Pore Complex -- Application Of STED Microscopy To Cell Biology Questions -- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes -- Direct Stochastic Optical Reconstruction Microscopy (dSTORM) -- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.