Special Review Article -- Therapeutic Inhibition of Angiogenesis -- Angiogenesis Protocols in Vivo -- Microscopic Assessment of Angiogenesis in Tumors -- In Vivo Matrigel Migration and Angiogenesis Assays -- Alginate Microbead Release Assay of Angiogenesis -- Disc Angiogenesis Assay -- Sponge Implant Model of Angiogenesis -- Hollow Fiber Assay for Tumor Angiogenesis -- Dorsal Skinfold Chamber Preparation in Mice -- Angiogenesis Assays Using Chick Chorioallantoic Membrane -- Corneal Assay for Angiogenesis -- Angiogenesis Protocols in Vitro -- Collagen Gel Assay for Angiogenesis -- Chemotaxis and Chemokinesis in 3D Macromolecular Matrices -- Rat Aortic Ring -- In Vitro Matrigel Angiogenesis Assays -- Associated Techniques -- Microvessel Endothelial Cells from Human Adipose Tissues -- Transfection and Transduction of Primary Human Endothelial Cells -- Vascular Smooth Muscle Cells -- Bovine Retinal Microvascular Pericytes.

Although angiogenesis-the formation of new blood vessels from pre-existing ones-has emerged as an exciting new target for the treatment of many varied disorders, fundamental aspects of this important biological process remain poorly understood. To address the needs of those working in such a dramatically advancing area, Cliff Murray and a panel of experienced investigators have brought together in Angiogenesis Protocols a comprehensive collection of key techniques for assessing the formation of new blood vessels in both whole tissues and cell culture. These readily reproducible methods range from low-cost in vitro assays to cutting-edge in vivo models, such as the dorsal window chamber, which demands high levels of surgical skill as well as relatively expensive, custom-made equipment. The authors have also included details of the associated techniques for the isolation, identification, and culture of microvessel endothelial cells from human adipose tissues and for the transfection and transduction of primary human endothelial cells. Timely and highly practical, Angiogenesis Protocols is a gold-standard resource for today's angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources.