Functional Glycomics and Glycobiology: An Overview -- Historical Overview of Glycoanalysis -- Quantitative Glycomics -- Human Gangliosides and Bacterial Lipo-oligosaccharides in the Development of Autoimmune Neuropathies -- Biotinylated Multivalent Glycoconjugates for Surface Coating -- Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry -- In Vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit -- Glycoprotein Characterization -- N-Linked Glycoprotein Analysis Using Dual-Extraction Ultrahigh-Performance Liquid Chromatography and Electrospray Tandem Mass Spectrometry -- Microarray-Based Study of Carbohydrate-Protein Binding -- The Application of NMR Spectroscopy to Functional Glycomics -- Metabolomics in Glycomics -- Characterization of Lipid-Linked Oligosaccharides by Mass Spectrometry -- A Glycomics Approach to the Discovery of Potential Cancer Biomarkers -- Extraction of Chondroitin/Dermatan Sulfate Glycosaminoglycans from Connective Tissue for Mass Spectrometric Analysis -- N-Linked Protein Glycosylation in a Bacterial System -- Characterization of Polysaccharides Using Mass Spectrometry for Bacterial Serotyping -- Is Permethylation Strategy Always Applicable to Protein N-Glycosylation Study?: A Case Study on the O-Acetylation of Sialic Acid in Fish Serum Glycans -- Bioinformatics in Glycomics: Glycan Characterization with Mass Spectrometric Data Using SimGlycan™.

The study of functional glycomics requires the continuous development of rapid and sensitive methods for the identification of glycan structures and integration to structure-function relationships. In Functional Glycomics: Methods and Protocols, a panel of world-renowned experts provide new developments and emerging glycomics techniques in the form of detailed protocols exploring the fundamental challenges and most cutting-edge novel applications. Written in the highly successful Methods in Molecular Biology™ series format, chapters in this volume present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Functional Glycomics: Methods and Protocols serves as an ideal reference for scientists working in biochemistry, molecular biology, cell biology, immunology, microbiology, and virology and a guide to appropriate techniques and the design of achievable research plans in this vital field.