Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse -- Genetic Analysis of Complex Traits -- Sequence-Based Detection of Single Nucleotide Polymorphisms -- Genomic Mismatch Scanning for the Mapping of Genetic Traits -- Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization -- Construction of a Bacterial Artificial Chromosome Library -- Contiguation of Bacterial Clones -- Mapping of Genomic Clones by Fluorescence In Situ Hybridization -- Map Integration -- Construction of Full-Length-Enriched cDNA Libraries -- Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping -- Preparation and Screening of High-Density cDNA Arrays with Genomic Clones -- Direct Selection of cDNAs by Genomic Clones -- Exon Trapping -- Sequencing Bacterial Artificial Chromosomes -- Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics -- Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning -- Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization -- Isolation of Differentially Expressed Genes by Representational Difference Analysis -- Expression Profiling and Isolation of Differentially Expressed Genes by Indexing-Based Differential Display -- Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos -- Expression Monitoring Using cDNA Microarrays -- Prediction of Protein Structure and Function by Using Bioinformatics -- Identification of Novel Genes by Gene Trap Mutagenesis -- Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice -- Genomic Analysis Utilizing the Yeast Two-Hybrid System -- Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle -- Retroviral-Mediated Gene Transduction -- Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods -- Ribozyme Gene Therapy.

Genome analysis is essential both to understanding the molecular bases of physiological processes and to the development of novel therapies for treating human diseases. In Genomics Protocols, an expert panel of internationally acclaimed researchers from academia, industry, and leading genome centers provides a comprehensive generic set of protocols for gene hunting and genome analysis. Drawing on emerging technologies in the fields of bioinformatics and proteomics, these protocols cover not only those traditionally recognized as genomics, but also early therapeutic approaches exploiting the potential of gene therapy. Highlights include methods for the analysis of differential gene expression, SNP detection, comparative genomic hybridization, and the functional analysis of genes, as well as the use of bioinformatics for gene identification and the prediction of protein function. Each method includes step-by-step instructions and invaluable notes that describe the quirks in a procedure and the little tricks that make all the difference to a successful outcome. Comprehensive and eminently practical, Genomics Protocols provides academic and pharmaceutical researchers alike with richly detailed accounts of the most up-to-date genomics techniques, including the emerging technologies from proteomics-all highly effective tools that will help investigators expand their research horizons and better understand gene function.