Antibody and Enzyme Conjugates -- Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies -- Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals -- Smart Polymer-Streptavidin Conjugates -- Conjugates of Peptides and Proteins to Polyethylene Glycols -- Chemical Production of Bispecific Antibodies -- Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties -- Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase -- Nucleic Acid Conjugates -- Fluorescent Sample Labeling for DNA Microarray Analyses -- High-Density Labeling of DNA for Single Molecule Sequencing -- Sequence-Specific DNA Labeling Using Methyltransferases -- Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications -- Covalent Coupling of DNA Oligonucleotides and Streptavidin -- Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates -- Synthesis of Peptide Nucleic Acid-Peptide Conjugates -- Glycosyl and Lipid Conjugates -- Protein Lipidation -- Synthesis of Lipidated Peptides -- In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins -- Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling -- Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments -- Subtilisin-Catalyzed Glycopeptide Condensation -- Biofunctionalization of Surfaces -- Peptide Nucleic Acid Microarrays -- Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles -- Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging.

Contemporary approaches to the synthesis of chemically modified proteins not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety, but also the effective use of recent developments in microarray technologies and nanobiotechnology. In Bioconjugation Protocols: Strategies and Methods, expert laboratorians update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, and materials science. These readily reproducible methods cover the preparation of protein conjugates using covalent and noncovalent conjugation, the synthesis of nucleic acid conjugates using a variety of labeling techniques, and approaches to semisynthetic conjugates of proteins. Additional chapters address the biofunctionalization of inorganic surfaces, including the on-chip synthesis of peptide nucleic acids to generate microarrays for the high-throughput analysis of RNA and DNA, gold nanoparticles, and carbon nanotube probes for atomic force microscopy. All the protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods offers both novice and experienced researchers access to the broad array of techniques needed to master the semisynthesis of functional biomolecular reagents for a wide variety of applications-ranging from novel biomedical diagnostics, to powerful new therapeutics, to advanced biomaterials.