Cell Types Contributing to Immunological Tolerance -- Frontiers of Immunological Tolerance -- Balancing Tolerance and Immunity -- Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow -- Genetic Modification of Dendritic Cells Through the Directed Differentiation of Embryonic Stem Cells -- Generation of Immunocompetent T Cells from Embryonic Stem Cells -- Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells -- Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines -- The Study of Immunological Tolerance In Vitro -- Thymus Organogenesis and Development of the Thymic Stroma -- Generation of a Tissue-Engineered Thymic Organoid -- Studying T-Cell Repertoire Selection Using Fetal Thymus Organ Culture -- Investigating Central Tolerance With Reaggregate Thymus Organ Cultures -- Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles -- Gene Expression Profiling of Dendritic Cells by Microarray -- SAGE Analysis of Cell Types Involved in Tolerance Induction -- Analyzing the Physicodynamics of Immune Cells in a Three-Dimensional Collagen Matrix -- The Study of Immunological Tolerance In Vivo -- Etiology of Autoimmmune Disease -- Animal Models of Spontaneous Autoimmune Disease -- Antigen-Based Therapy and Immune-Regulation in Experimental Autoimmune Encephalomyelitis -- Induction and Regulation of Inflammatory Bowel Disease in Immunodeficient Mice by Distinct CD4+ T-Cell Subsets -- In Vivo Models for the Study of Transplantation Tolerance -- Ectopic Transplantation of Tissues Under the Kidney Capsule -- Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node -- Tracing Tolerance and Immunity In Vivo by CFSE-Labeling of Administered Cells -- Methods for Inducing and Breaking Immunological Tolerance -- Thymic Involution -- Inducing Mixed Chimerism and Transplantation Tolerance Through Allogeneic Bone Marrow Transplantation With Costimulation Blockade -- Induction of Dominant Tolerance Using Monoclonal Antibodies -- Induction of Tolerance by Adoptive Transfer of Treg Cells -- Modulation of the Immune Response Using Dendritic Cell-Derived Exosomes -- Breaking Self-Tolerance to Tumor-Associated Antigens by In Vivo Manipulation of Dendritic Cells.

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.