Overviews -- Light, Photoreceptors, and Circadian Clocks -- Statistical Analysis of Biological Rhythm Data -- Rhythmic Readouts -- Rhythmic Conidiation in Neurospora crassa -- Monitoring and Analyzing Drosophila Circadian Locomotor Activity -- Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms -- Locomotor Activity in Rodents -- Analysis of Circadian Leaf Movement Rhythms in Arabidopsis thaliana -- Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria -- Analysis of Rhythmic Gene Expression in Adult Drosophila Using the Firefly Luciferase Reporter Gene -- Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes -- Identification of Clock Genes -- Specialized Techniques for Site-Directed Mutagenesis in Cyanobacteria -- Novel Strategies for the Identification of Clock Genes Neurospora With Insertional Mutagenesis -- Mutagenesis With Drosophila -- Mutagenesis in Arabidopsis -- Yeast Two-Hybrid Screening -- Microarrays -- Microarrays -- Identification of Clock Genes Using Difference Gel Electrophoresis -- Gene Expression: RNA -- Isolation of Total RNA From Neurospora Mycelium -- RNA Extraction From Drosophila Heads -- Extraction of Plant RNA -- RNA Extraction From Mammalian Tissues -- Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa -- RNase Protection Assay -- Quantitative Polymerase Chain Reaction -- Gene Expression: Proteins -- Protein Extraction, Fractionation, and Purification From Cyanobacteria -- Protein Extraction From Drosophila Heads -- Plant Protein Extraction -- Protein Extraction From Mammalian Tissues -- Western Blotting -- Coimmunoprecipitation Assay -- In Vitro Phosphorylation and Kinase Assays in Neurospora crassa -- In Vitro Systems -- Basic Protocols for Drosophila S2 Cell Line -- Coimmunoprecipitation on Drosophila Cells in Culture -- Basic Protocols for Zebrafish Cell Lines -- Manipulation of Mammalian Cell Lines for Circadian Studies -- Reporter Assays -- Use of Firefly Luciferase Activity Assays to Monitor Circadian Molecular Rhythms In Vivo and In Vitro -- Suprachiasmatic Nucleus Cultures That Maintain Rhythmic Properties In Vitro -- Microscopy Analysis -- RNA In Situ Hybridizations on Drosophila Whole Mounts -- In Situ Hybridization of Suprachiasmatic Nucleus Slices -- Immunohistochemistry in Drosophila -- Immunocytochemistry on Suprachiasmatic Nucleus Slices -- Immunofluorescence Analysis of Circadian Protein Dynamics in Cultured Mammalian Cells.

The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems.