Generation of Prostate Cancer Patient-derived Xenografts to Investigate Mechanisms of Novel Treatments and Treatment Resistance -- Methods to Study Angiogenesis in a Mouse Model of Prostate Cancer -- Methodologies Applied to Establish Cell Cultures in Prostate Cancer -- Protocols for Migration and Invasion Studies in Prostate Cancer -- Transplantable Animal Studies and Whole-body Optical Imaging in Prostate Carcinoma -- Protocols for Tissue Microarrays in Prostate Cancer Studies -- Functional Studies on Steroid Receptors -- Protocols for Studies on TMPRSS/ERG in Prostate Cancer -- Protocols for the Study of Taxanes Chemosensitivity in Prostate Cancer -- A Method for Prostate and Breast Cancer Cell Spheroid Cultures using Gelatin Methacryloyl-based Hydrogels -- Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer -- Protocols for Studies on Stromal Cells in Prostate Cancer -- Techniques for Evaluation of AR Transcriptional Output and Recruitment to DNA -- NMR-based Prostate Cancer Metabolomics -- Studies on Steroid Receptor Coactivators in Prostate Cancer.

This volume presents methods and protocols on modern analysis of steroid receptor and prostate cancer function. Chapters detail androgen, androgen receptor action, anti-androgen enzalutamide, in vitro and in vivo models, imaging, gene fusions, and help facilitate studies with novel drugs including anti-androgens and chemotherapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Prostate Cancer: Methods and Protocols aims to provide easily reproducible techniques.