Using QSAR Models to Predict Mitochondrial Targeting by Small Molecule Xenobiotics within Living Cells -- DQAsomes as the Prototype of Mitochondria-targeted Pharmaceutical Nanocarriers: An Update -- Synthesis and Characterization of Mitochondria-Targeted Triphenyltriphenylphosphonium Bolaamphiphiles -- Synthesis and Evaluation of 18F-labeled Fluoroalkyl Triphenylphosphonium Salts as Mitochondrial Voltage Sensors in PET Myocardial Imaging -- Novel Mitochondria-Targeted Triphenylphosphonium Conjugates of Linear beta-Phosphorylated Nitrones: Preparation, 31P NMR Mitochondrial Distribution, EPR Spin Trapping Reporting, and Site-Directed Anti-Apoptotic Properties -- Insights on Targeting Small Molecules to the Mitochondrial Matrix and the Preparation of MitoB and MitoP as exomarkers of Mitochondrial Hydrogen Peroxide -- Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes -- Imaging Mitochondrial Hydrogen Peroxide in Living Cells -- Synthesis and Testing Of Novel Isomeric Mitochondriotropic Derivatives of Resveratrol and Quercetin -- Bridging the Gap between Nature and Antioxidant Setbacks: Delivering Gallic Acid to Mitochondria -- Liposomal Delivery of Cyclocreatine Impairs Cancer Cell Bioenergetics Mediating Apoptosis -- Mt-fura-2, a ratiometric Mitochondria-targeted Ca2+ sensor. Determination of spectroscopic properties and Ca2+ Imaging Assays -- Sequence-Specific Control of Mitochondrial Gene Transcription using Programmable Synthetic Gene Switches called MITO-PIPs -- Targeting the Mitochondrial Genome via a MITO-Porter: Evaluation of mtDNA and mtRNA Levels and Mitochondrial Function -- mTRIP, an Imaging Tool to Investigate Mitochondrial DNA dynamics in Physiology and Disease at the Single-Cell Resolution -- Development of Mitochondria-Targeted Imaging Nanoplatforms by Incorporation of Fluorescent Carbon Quantum Dots -- Norbormide-based Probes and their Application for Mitochondrial Imaging in Drosophila Melanogaster -- Live-cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine -- Protein Supercomplex Recording In Living Cells Via Position-Specific Fluorescence Lifetime Sensors -- Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid -- Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry -- Janus-type Mesoporous Silica Nanoparticles for Sequential Tumoral Cell and Mitochondria Targeting -- Split Green Fluorescent Protein-Based Contact Site Sensor (SPLICS) for Heterotypic Organelle Juxtaposition as Applied to ER - Mitochondria Proximities -- Qualitative Characterization of the Rat liver Mitochondrial Lipidome using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS -- Live Imaging of Mitochondria in Kidney Tissue -- Time-resolved Imaging of Mitochondrial Flavin Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cardiac Cells -- Mito-SinCe2 Approach to Analyze Mitochondrial Structure-Function In Single Cells -- Computer-aided Prediction of Protein Mitochondrial Localization.

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume I guides readers through chapters on QSAR models, DQAsomes, synthesis of Triphenylphosphonium Phospholipid Conjugates, testing of novel isomeric mitochondriotropic derivatives, mTRIP, mitochondria-targeted imaging nanoplatforms, live-cell assessment, mitochondrial coenzyme Q10, rat liver Mitochondrial Lipidome, and Mito-SinCe2. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 1: Targeting Mitochondria aims to be a comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.