Advances in Confocal Microscopy and Selected Applications -- Choosing Fluorescent Probes and Labeling Systems -- General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy -- Microfabricated devices for confocal microscopy on biological samples -- Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging -- High-resolution multicolor imaging of mitochondria in lymphocytes -- Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy -- Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy -- Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight -- Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum -- Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis -- High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains -- Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy -- Studying Neuronal Biology Using Spinning Disc Confocal Microscopy -- Method for Acute Intravital Imaging of the Large Intestine in Live Mice -- Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism -- Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry -- FLIM Imaging for Metabolic Studies in Live Cells -- A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM).

This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types. Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.