Stress and Systemic Inflammation: Yin-Yang Dynamics in Health and Diseases -- Intersections between Neuroimmune-Microbiota -- Psychoneuroimmunology: The Experiential Dimension -- Ecological Context and Human Variation: Applying the Principles of Biological Anthropology to Psychoneuroimmunology -- Neuroimmune Imbalances and Yin-Yang Dynamics in Stress, Anxiety, and Depression -- Increasing Resilience to Traumatic Stress: Understanding the Protective Role of Well-Being -- Exploring the Diagnostic Potential of Immune Biomarker Co-Expression in Gulf War Illness -- Breaking Away: The Role of Homeostatic Drive in Perpetuating Depression -- Neuroimmune Mechanisms of Depression in Adults with Heart Failure -- How To Monitor the Neuroimmune Biological Response in Patients Affected by Immune Alteration-Related Systemic Diseases -- Application of Chemogenetics and Optogenetics to Dissect Brain-Immune Interactions -- Psychoneuroimmunology and Natural Killer Cells: The Chromium Release Whole Blood Assay -- Mouse Testing Methods in Psychoneuroimmunology 2.0: Measuring Behavioral Responses -- The MRL Model: A Valuable Tool in Studies of Autoimmunity-Brain Interactions -- PET Imaging in Psychoneuroimmunology Research -- The Vaccination Model in Psychoneuroimmunology Research: A Review -- Measuring Vaccine Responses in the Multiplex Era -- Sculpting the Sculptors: Methods for Studying the Fetal Cholinergic Signaling on Systems and Cellular Scales -- Perinatal Psychoneuroimmunology: Protocols for the Study of Prenatal Stress and Its Effects on Fetal and Postnatal Brain Development.

This fully-updated book provides systems-based methodologies and innovative technologies that can be used for solving complicated problems of complex systems. A wide range of theoretical and experimental approaches are introduced for practical applications, as the content explores the basic and novel concepts in psychoneuroimmunology (PNI), especially the relationships among stress, inflammation, and psychophysiological disorders, as well as various cutting-edge technologies and methods for PNI studies, including the utilizations of mouse models and vaccine models. In addition, this book focuses on translational medicine by applying PNI methods in clinical practice. As part of the Methods in Molecular Biology series, the chapters seek to provide a practical, state-of-the-art, and holistic view for the translation of PNI into better preventive and personalized medical practice. Authoritative and hands-on, Psychoneuroimmunology: Methods and Protocols, Second Edition will be of use to biomedical students and professionals at all levels who are interested in integrative studies in psychology, psychiatry, neuroscience, immunology, molecular biology, genetics, bioengineering, physiology, pathology, microbiology, systems biology, and clinical medicine.