Worldwide Clinical Demand for Antibiotics: Is It a Real Countdown? -- Collection, Extraction, and In Vitro Antibacterial Evaluation of Plants Used in Traditional Medicine -- Microbial Isolation and Characterization of New Antibiotic‑Producer Strains from Decayed Wood -- Characterization of Extremophilic Actinomycetes Strains as Sources of Antimicrobial Agents -- Dilution-To-Extinction Platform for the Isolation of Marine Bacteria Producing Antitumor Compounds -- Screening Systems for Stable Markerless Genomic Deletions/Integrations in Streptomyces Species -- Antibacterial Assay in a Screening Project -- Screening of Antibiotic Gene Clusters in Microorganisms Isolated from Wood -- Methods in Screening Antiviral Drugs Against Enterovirus 71 -- High-Efficiency Electroporation for Genetic Improvement of Fungal Strains -- Penicillium chrysogenum Fermentation and Analysis of Benzylpenicillin by Bioassay and HPLC -- Screening Fermentation and Extract Generation -- A Simple Antifungal Assay for Testing Actinomycetes and Other Microbial Extracts -- A User Guide for the Identification of New RiPP Biosynthetic Gene Clusters Using a RiPPER-Based Workflow -- Novel Methods to Identify Oxidative Stress-Producing Antibiotics -- Coupled Transcriptomics for Differential Expression Analysis and Determination of Transcription Start Sites: Design and Bioinformatics -- High Throughput Screening Assays for Dengue Antiviral Drug Development -- Molecular Biology Methods in Streptomyces rimosus, a Producer of Oxytetracycline -- Production, Detection, Extraction, and Quantification of Polyene Antibiotics -- Strain Improvement Program of Streptomyces roseosporus for Daptomycin Production -- Comparative Metabolomics Revealing the Metabolic Responses of Pathogenic Bacteria to Different Antibiotics -- Proteomic Analysis of Antibiotic Susceptibility in Enterobacteriaceae -- Measurement of the Intracellular Mycobacterium tuberculosis Drug Effect and Prediction of the Clinical Dose-Response Relationship Using Intracellular Pharmacodynamic Modeling (PDi) -- Clinical Drug Trials: The Path to the Patient -- Antibiotics Therapy in the Multi-Resistant Patient.

Antimicrobial resistance will become a global health threat since antimicrobial treatments continue at the forefront of the defense against microbial infections. To respond to the issue, this detailed book explores vital methodologies currently in use to advance our understanding of antibiotic issues and answer the worldwide demand for novel antibiotics therapies. Beginning with a review chapter that guides the reader through the worldwide demand for novel antibiotics therapies, the volume continues with sections covering new screening procedures and environmental sources, advances in analytical, microbiological, and biotechnological methodologies, antibiotic production and antibiotic resistances, as well as considerations of drug trials and clinical concerns regarding multi-resistant patients. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antimicrobial Therapies: Methods and Protocols provides a reference source for health, laboratory, and industrial professionals, as well as for graduate students in a number of bio-sanitary disciplines, including medicine, nursery, biotechnology, veterinary, microbiology, genetics, molecular biology, nutrition, farming, and more. Chapter "14" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.