Principles and Practices of DNA Analysis : A Laboratory Manual for Forensic DNA Typing / by Hirak Ranjan Dash, Pankaj Shrivastava, Surajit Das
By: Dash, Hirak Ranjan, autor
Contributor(s): Shrivastava, Pankaj, autor
| Das, Surajit., autor
Material type:
E-bookSeries: (Springer Protocols Handbooks, 1949-2456).Publisher: New York, NY : Springer International Publising, 2020Edition: 1st edition 2020.Description: 1 recurso en línea (XIX, 344 páginas) : 107 ilustraciones, 76 ilustraciones a color.ISBN: 9781071602744.Subject: ADN -- Análisis -- Manuales de laboratorio
| Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
|---|---|---|---|---|---|---|---|---|
LIBRO-E NO PRÉSTAMO
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Madrid Digital Acceso Electrónico (UEM) | Ciencias de la Salud | QP264 2020 EB (Browse shelf(Opens below)) | Acceso electrónico | eBook.20122532 |
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Module 1_Overview of forensic DNA analysis -- Module 2_Manual Extraction of DNA from biological samples -- Module 3_Automatic and semi-automatic extraction of DNA from biological samples -- Module 4_Qualitative and quantitative assessment of extracted DNA -- Module 5_Amplification of various STR markers using multiplex PCR.-Module 6_Genotyping and analysis of Results -- Module 7_Case studies -- Module 8_Advanced technologies for forensic DNA analysis. .
The book presents hands-on protocols for conventional and advanced forensic DNA fingerprinting experiments. It includes manual, semi-automatic, and advanced automatic techniques for DNA extraction from different biological samples. It also discusses various qualitative and quantitative approaches for the assessment of extracted forensic DNA. It contains protocols for the amplification of short tandem repeat markers (STRs) for the amplification-based target enrichment of the forensic samples. Further, it examines genotyping of the STR loci through capillary electrophoresis and includes real-world case studies where forensic DNA analysis has been used in the criminal and civil disputes. The book concludes by presenting technological developments in the field of DNA forensic analysis. Suitable for beginners, it is a key reference resource on a wide variety of DNA profiling techniques and applications. .
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