Regulatory Non-coding RNAs: An Overview -- RNA Precipitation -- Quantitative and Qualitative Assessment of Small RNA Preparations -- Non-coding RNA Silencing in Mammalian Cells by Antisense LNA GapmeRs Transfection -- Northern Blot Detection of Tiny RNAs -- Stem-loop qRT-PCR Based Quantification of miRNAs -- Detection and Labeling of Small Non-coding RNAs by Splinted Ligation -- Fluorescence In Situ Hybridization Of Small Non-coding RNAs -- Using native RIP, UV-CLIP or fCLIP to Address Protein-RNA Interactions In Vivo -- Purification of RiboNucleoProtein Particles by MS2-MBP Affinity Chromatography -- Study of genome-wide occupancy of long non-coding RNAs using Chromatin Isolation by RNA Purification (ChIRP) -- Gene Reporter Assays to Study miRNA Function -- CRISPR/Cas9 System to Knock-Down microRNA In Vitro and In Vivo -- Microarray Analysis of Whole-Transcriptome RNAs including Non-Coding RNAs -- Isolation, Extraction, and Deep-Sequencing Analysis Of Extracellular RNAs (exRNAs) from Human Plasma -- Analysis of the RNA and protein complexome by Grad-seq -- µIVC-Seq: A Method for Ultrahigh-throughput Development and Functional Characterization of Small RNAs -- RNA Secondary Structure Study by Chemical Probing Methods using DMS and CMCT -- Application of NMR Spectroscopy to Determine the 3D structure of Small Non-coding RNAs.

This second edition provides new and updated chapters detailing methods tackling all aspects of small non-coding RNAs biology. Chapters guide readers through customized dedicated protocols and technologies that will help decipher the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Small Non-Coding RNAs: Methods and Protocols, Second Edition aims to be a valuable help and of great interest to a large number of researchers working in the field of RNA or willing to develop RNA studies.