Chemical Genetics to Uncover Mechanisms Underlying Lipid-Mediated -- Signaling Events in Plants -- Method for Phenotypic Chemical Screening to Identify Cryptochrome Inhibitor -- Whole Seedling Based Chemical Genetic Screens in Arabidopsis -- Identification of Type iii Secretion Inhibitors for Plant Disease Management -- Investigation of Drug Efficacy by Screening Bioactive Chemical Effects on Plant Cell Subcellular Architecture -- In Vivo Chemical Genomics with Random Cyclized Peptides -- Interfering Peptides Targeting Protein-Protein Interactions in the Ethylene Plant Hormone Signaling Pathway as Tools to Delay Plant Senescence -- The Screening for Novel Inhibitors of Auxin-Induced Ca2+ Signaling -- Identification of ABA Receptor Agonists Using a Multiplexed High-Throughput Chemical Screening -- A Luciferase Reporter Assay to Identify Chemical Activators of ABA Signaling -- Identification of Novel Molecular Regulators Modulating Ethylene Biosynthesis Using EMS-Based Genetic Screening -- Investigation of Auxin Biosynthesis and Action Using Auxin Biosynthesis Inhibitors -- Target Profiling of an Anticancer Drug Curcumin by an In Situ Chemical Proteomics Approach -- Label-Free Target Identification and Confirmation Using Thermal Stability Shift Assays -- Drug Affinity Responsive Target Stability (DARTS) Assay to Detect Interaction Between a Purified Protein and a Small Molecule -- Using Differential Scanning Fluorimetry (DSF) to Detect Ligand Binding with Purified Protein -- Microscale Thermophoresis (MST) to Detect the Interaction between Purified Protein and Small Molecule.

This second edition volume is a companion volume to the previous edition and looks at new findings on novel bioactive chemicals and cognate targets, as well as the use of synthetic small molecules and a variety of tools to understand these processes. Chapters in this book cover topics such as screening plants for novel bioactive chemicals including lipid signaling and photo receptors; small molecule screens to include peptide ligands to generate new variations; using new chemicals to affect and dissect hormone signaling; and the application of easier microscale methods to simplify target identification and validation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Plant Chemical Genomics: Methods and Protocols, Second Edition is a valuable resource that provides a foundation of techniques for novice and expert researchers in the plant chemical biology community.