Chapter 1. Enzyme Linked Immuno sorbent Assay Detection of Bacterial Wilt Causing Ralstonia solanacearum -- Chapter 2. Diagnosis of Tropical Fascioliasis using Specific Antigen Detection in Faeces of Infected Human Being or Animal Species -- Chapter 3. CMV Promoter‐Driven Expression and Visualization of Tagged Proteins in Live and Fixed Zebrafish Embryonic Epidermis -- Chapter 4. Detection of Blood Parasites and Estimation of Haematological Indices in Fish -- Chapter 5. Techniques to Conduct Morphological and Molecular Investigations of Nematodes -- Chapter 6. Computational Approaches in Drug Designing and its Application -- Chapter 7. Bacteriophage Control for Pseudomonas Aeruginosa Biofilm Formation and Eradication -- Chapter 8 -- Detection of Phospho-proteins (phospho-serine and phospho-threonine) from Thylakoid Membranes using Western Blotting -- Chapter 9. Estimation of Plasma membrane and Tonoplast ATPase activity in Plant Tissues -- Chapter 10. Quantification of Conserved MicroRNA in Plants and Validation of New Targets -- Chapter 11. Isolation of Natural Killer Cells from Mouse Bone Marrow and Spleen -- Chapter 12. Gene Expression Analysis from Human Peripheral Blood Mononuclear Cells -- Chapter 13. Use of Droplet PCR in Biomedical Research -- Chapter 14. Determination of Michaelis-Menten Enzyme Kinetics parameters of Alkaline Phosphatase in clinical sample -- Chapter 15. Novel Technique for the Detection of LncRNAs on Tissue Sections.

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. .