Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview -- Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase -- High-Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins -- Detecting Endogenous Rab8 Activation -- Testing the Phenotypic Effects of a Rab Chimera that Resolves Exchange Factor Specificity from Effector Specificity -- Profiling Structural Alterations during Rab5 Nucleotide Exchange by HDX-MS -- CLEM Characterization of Rab8 and Associated Membrane Trafficking Regulators at Primary Cilium Structures -- Imaging of Spatial Cycling of Rab GTPase in the Cell -- Deconvolution of Multiple Rab Binding Domains using the Batch Yeast 2-Hybrid Method DEEPN -- Determination of the Rab27-Effector Binding Affinity Using a High-Throughput FRET-Based Assay -- Methods to Study the Unique SOCS Box Domain of the Rab40 Small GTPase Subfamily -- Using GBP Nanotrap to Restore Autophagy in the Rab5/Vps21 Mutant by Forcing Snf7 and Atg17 Interaction -- Establishing Regulation of a Dynamic Process by Ypt/Rab GTPase: A Case for Cisternal Progression -- Methods for Assessing the Regulation of a Kinase by the Rab GTPase Ypt1 -- Qualitative and Quantitative Assessment of the Role of Endocytic Regulatory and/or Rab Proteins in Mitochondrial Fusion and Fission -- Characterization of the Role of Rab18 in Mediating LD-ER Contact and LD Growth -- Methods for Establishing Rab Knockout MDCK Cells -- Generating Rab6 Conditional Knockout Mice -- Use of Immunohistochemistry to Determine Expression of Rab5 Subfamily of GTPases in Mature and Developmental Brains -- Assessing Rab5 Activation in Health and Disease -- Quantitative Fluorescence Microscopy for Detecting Mammalian Rab Vesicles within the Parasitophorous Vacuole of the Human Pathogen Toxoplasma gondii.

This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Rab GTPases: Methods and Protocols, Second Edition is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond.