Coronaviruses: An Updated Overview of Their Replication and Pathogenesis -- Discovery of Novel Coronaviruses in Rodents -- Detection and Discovery of Coronaviruses in Wild Bird Populations -- Competitive ELISA for the Detection of Serum Antibodies Specific for Middle East Respiratory Syndrome-Coronavirus (MERS-CoV) -- Whole Genome Sequencing Protocols for IBV and Other Coronaviruses Using High-Throughput Sequencing -- Isolation and Tissue Culture Adaptation of Porcine Deltacoronavirus: A Case Study -- The Preparation of Chicken Kidney Cell Cultures for Virus Propagation -- The Preparation of Chicken Tracheal Organ Cultures and Their Application for Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation -- Isolation and Propagation of Coronaviruses in Embryonated Eggs -- Well-Differentiated Primary Mammalian Airway Epithelial Cell Cultures -- Quantification of Coronaviruses by Titration In Vitro and Ex Vivo -- Transient Dominant Selection for the Modification and Generation of Recombinant Infectious Bronchitis Coronaviruses -- In-Yeast Assembly of Coronavirus Infectious cDNA Clones Using a Synthetic Genomics Pipeline -- Proximity Labeling for Identification of Coronavirus-Host Protein Interactions -- Using Yeast to Identify Coronavirus-Host Protein Interactions -- Determining How Coronaviruses Overcome the Interferon and Innate Immune Response -- Ribopuromycylation in Coronavirus-Infected Cells -- Visualizing Coronavirus Entry into Cells -- Preparation of Cultured Cells Using High Pressure Freezing and Freeze Substitution for Subsequent 2D or 3D Visualization in the Transmission Electron Microscope.

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.