Enzyme Biosensors -- Principles of Enzyme Biosensors -- Enzyme Biosensors Based on pH Electrode -- Enzyme Biosensors Based on Gas Electrodes -- Enzyme Biosensors Based on ISFETs -- Enzyme Biosensors Based on Oxygen Detection -- Enzyme Biosensors Based on the Hydrogen Peroxide Electrode -- Enzyme Biosensors Based on Mediator-Modified Carbon Paste Electrode -- Enzyme Biosensors Based on Electron Transfer Between Electrode and Immobilized Peroxidases -- Enzyme Biosensors Based on Redox Polymers -- Enzyme Biosensors Based on Metallized Carbon Electrodes -- Enzyme Biosensors Based on Conducting Polymers -- Enzyme Sensors Based on Conductimetric Measurement -- Enzyme Biosensors Based on Thermal Transducer/Thermistor -- Enzyme Biosensors Based on Fluorometric Detection -- Microbial Biosensor -- Microbial biosensors based on oxygen electrodes -- Microbial biosensors based on respiratory inhibition -- Microbial biosensors based on potentiometric detection -- Microbial biosensors based on optical detection.

In Enzyme and Microbial Biosensors: Protocols and Techniques, master experimentalists detail cutting-edge methods developed in their own laboratories for the construction and evaluation of enzyme- and cell-based biosensors. The enzyme biosensors detailed here are based on potentiometric, amperometric, conductimetric, optical, and thermal transducers, whereas the microbial biosensors are based on oxygen-gas electrode and optical techniques in which microorganisms are coupled to the signal transducer. All the techniques are thoroughly tested and are presented in sufficient detail to ensure robust and easily reproducible results. A companion volume, Affinity Biosensors: Protocols and Techniques, by Rogers & Mulchandani, concentrates on affinity biosensors based on optical, electrochemical, thermal, acoustic, and plasmon resource techniques as applied to nucleic acids, liposomes, and eukaryotic cells. Clearly the optimal starting point for all graduate students, postdoctoral and senior researchers, and technicians in academia, industry, and research establishments seeking rapid entry into the field of biosensors, Enzyme and Microbial Biosensors: Protocols and Techniques instantly becomes today's leading reference. Its step-by-step descriptions of various enzyme- and microbial-based biosensor techniques will allow both novices and experienced investigators alike successfully to apply these powerful new tools, and thus sharply enhance their laboratories' productivity.