Detection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-like Receptors -- Nucleic Acid Recognition in Dendritic Cell -- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems -- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro -- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes -- Mapping of Optimal CD8 T Cell Epitopes -- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection -- MicroRNA Methodology: Advances in miRNA-technologies -- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) -- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus -- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice -- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice -- Bifunctional siRNAs for Tumor Therapy.

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.