Protocol for Extraction and Isolation of Brassinosteroids from Plant Tissues -- Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye -- Physiological Analysis of Brassinosteroid Responses and Sensitivity in Rice -- Light Regulation of Brassinosteroid Signaling Components: Checking Regulation of Protein Stability in Darkness -- Approaches to Study Light Effects on Brassinosteroid Sensitivity -- A Technical Framework for Studying the Signaling Nexus of Brassinosteroids and Immunity -- Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing -- Quantitation of Cell Type-Specific Responses to Brassinosteroid by Deep Sequencing of Polysome-Associated Polyadenylated RNA -- Methods for Modeling Brassinosteroid-Mediated Signaling in Plant Development -- Quantitative Microscopic Analysis of Plasma Membrane Receptor Dynamics in Living Plant Cells -- Analysis of In Vitro DNA Interactions of Brassinosteroid-Controlled Transcription Factors Using Electrophoretic Mobility Shift Assay -- Identification of Brassinosteroid Signaling Complexes by Coimmunoprecipitation and Mass Spectrometry -- Simplified Enrichment of Plasma Membrane Proteins from Arabidopsis thaliana Seedlings Using Differential Centrifugation and Brij-58 Treatment -- Probing Activation and Deactivation of the BRASSINOSTEROID INSENSITIVE1 Receptor Kinase by Immunoprecipitation -- The Primary Root of Sorghum bicolor (L. Moench) as a Model System to Study Brassinosteroid Signaling in Crops -- Brassinosteroid Action in Plant Abiotic Stress Tolerance.

This detailed volume compiles state-of-the-art methodologies for the study of brassinosteroid hormones, contributed by recognized researchers in the field, in order to bring together different experimental and theoretical biology techniques for addressing the questions of how brassinosteroids function in Arabidopsis and other agriculturally valuable species, such as rice and sorghum. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Brassinosteroids: Methods and Protocols assesses a wide range of appealing methodologies for experts as well as researchers with a general interest in hormone signaling in plants.