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DNA and RNA Isolation Techniques for Non-Experts / by Akash Gautam

By: Gautam, Akash, autor
Material type: materialTypeLabelE-bookSeries: (Techniques in Life Science and Biomedicine for the Non-Expert, 2367-1122).Publisher: Cham : Springer International Publishing, 2022Edition: First edition 2022.Description: 1 recurso en línea (XVI, 203 páginas) : 58 ilustraciones, 48 ilustraciones a color.ISBN: 9783030942304.Subject: NucleótidosOnline resources: Acceso a este recurso digital (usuarios Universidad Europea de Madrid)Digital Resources
Contents:
1-Basic Idea About Nucleic Acids For Non-Experts -- 2-General Steps During Isolation Of DNA And RNA -- 3-Phenol-Chloroform DNA Isolation Method -- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method -- 5-Spin Column-Based Isolation Of Nucleic Acid -- 6-Isolation Of Plasmid DNA By Alkaline Lysis -- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment -- 8-Lithium Chloride Based Isolation Of DNA And RNA -- 9-Isolation Of Bacteriophage DNA By Peg Method -- 10-DNA Isolation By Chelex Method -- 11-DNA Isolation By Lysozyme And Proteinase K -- 12-Salting-Out Method For Blood Samples -- 13-CTAB Or SDA Based Isolation Of Plant's DNA -- 14-DNA Extraction By Spooling Method -- 15-Magnetic Bead-Based Nucleic Acid Isolation -- 16-Density Gradient-Based Nucleic Acid Isolation -- 17-DNA Extraction From Agarose Gel Though Paper-Strips -- 18-Transformation Or Genetic Modification Of Cells/Organisms -- 19-Gene Cloning And Vectors -- 20-Polymerase Chain Reaction (PCR) -- 21-Southern And Northern Blotting -- 22-Genome Mapping -- 23-Applications Of DNA Sequencing Technologies For Current Research.
In: Springer Nature eBookSummary: This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.
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Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
LIBRO-E NO PRÉSTAMO LIBRO-E NO PRÉSTAMO Madrid Digital Acceso Electrónico (UEM) FCBS | FCAD QP625 .N89 2022 EB (Browse shelf(Opens below)) Acceso electrónico eBook.09092176
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1-Basic Idea About Nucleic Acids For Non-Experts -- 2-General Steps During Isolation Of DNA And RNA -- 3-Phenol-Chloroform DNA Isolation Method -- 4-RNA Isolation By The Guanidinium-Acid-Phenol Method -- 5-Spin Column-Based Isolation Of Nucleic Acid -- 6-Isolation Of Plasmid DNA By Alkaline Lysis -- 7-DNA Isolation By Hydrophilic Ionic Liquid Treatment -- 8-Lithium Chloride Based Isolation Of DNA And RNA -- 9-Isolation Of Bacteriophage DNA By Peg Method -- 10-DNA Isolation By Chelex Method -- 11-DNA Isolation By Lysozyme And Proteinase K -- 12-Salting-Out Method For Blood Samples -- 13-CTAB Or SDA Based Isolation Of Plant's DNA -- 14-DNA Extraction By Spooling Method -- 15-Magnetic Bead-Based Nucleic Acid Isolation -- 16-Density Gradient-Based Nucleic Acid Isolation -- 17-DNA Extraction From Agarose Gel Though Paper-Strips -- 18-Transformation Or Genetic Modification Of Cells/Organisms -- 19-Gene Cloning And Vectors -- 20-Polymerase Chain Reaction (PCR) -- 21-Southern And Northern Blotting -- 22-Genome Mapping -- 23-Applications Of DNA Sequencing Technologies For Current Research.

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.

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