Chapter 1. Introduction to metabolic engineering -- Chapter 2. Microbial strain engineering -- Chapter 3. Techniques for detection and extraction of metabolites -- Chapter 4. Genetically encoded biosensors and their applications in the development of microbial cell factories -- Chapter 5. Metabolic products of mixed culture fermentation -- Chapter 6. Recent advances in genetic engineering tools for metabolic engineering -- Chapter 7. Recent developments in synthetic biology toolbox -- Chapter 8. Ethical, patent and regulatory issues in microbial engineering -- Chapter 9. Microbial production of Vitamins -- Chapter 10. Bacterial production of organic acids and subsequent metabolism -- Chapter 11. Microbial production of polysaccharides -- Chapter 12. Microbial Production of Industrial Proteins and Enzymes Using Metabolic Engineering -- Chapter 13. Microbial production of antibiotics using metabolic engineering -- Chapter 14. Metabolic Engineering Opening New Avenues for Therapeutics -- Chapter 15. Biofuels Production Using Metabolic Engineering -- Chapter 16. Microbial Utilization of Glycerol for Biomanufacturing -- Chapter 17. Revealing Function of Amino Acids in Nitrifying and Anammox Systems through Chromatography and Metagenomic Analyses.-.

This book provides a comprehensive overview of the basic and advanced metabolic engineering technologies used to generate natural metabolites and industrially important biomolecules. Metabolic engineering has the potential to produce large quantities of valuable biomolecules in a renewable and sustainable manner by extending or modifying biosynthetic pathways in a wide range of organisms. It has been successfully used to produce chemicals, drugs, enzymes, amino acids, antibiotics, biofuels, and industrially important pharmaceuticals. The book comprehensively reviews the various metabolites detection, extraction and biosensors and the metabolic engineering of microbial strains for the production of industrially useful enzymes, proteins, organic acids, vitamins and antibiotics, therapeutics, chemicals, and biofuels. It also discusses various genetic engineering and synthetic biology tools for metabolic engineering. In closing, the book discusses ethical, patenting and regulatory issues in the metabolic engineering of microbes. This book is a valuable source not only for beginners in metabolic engineering, but also students, researchers, biotechnology and metabolic engineering based company.