MARC details
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| 001 - NÚMERO DE CONTROL |
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392327 |
| 003 - IDENTIFICADOR DEL NÚMERO DE CONTROL |
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ES-MaUEC |
| 005 - FECHA Y HORA DE LA ÚLTIMA TRANSACCIÓN |
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20230908095353.0 |
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100301s2006 xxu| o |||| 0|eng d |
| 020 ## - NÚMERO INTERNACIONAL ESTÁNDAR DEL LIBRO |
|---|
| Número Internacional Estándar del Libro |
9781592599981 |
| 024 7# - IDENTIFICADOR DE OTROS ESTÁNDARES |
|---|
| Número estándar o código |
10.1385/1592599982 |
| Fuente del número o código |
doi |
| 040 ## - FUENTE DE LA CATALOGACIÓN |
|---|
| Centro catalogador/agencia de origen |
ES-MaUEC |
| Lengua de catalogación |
spa |
| Centro/agencia transcriptor |
ES-MaUEC |
| Centro/agencia modificador |
ES-MaUEC |
| 050 #4 - SIGNATURA TOPOGRÁFICA DE LA BIBLIOTECA DEL CONGRESO |
|---|
| Número de clasificación |
QP601.7 |
| Número de documento/Ítem |
2006 EB |
| 245 00 - MENCIÓN DE TÍTULO |
|---|
| Título |
Cytochrome P450 Protocols |
| Mención de responsabilidad, etc. |
edited by Ian R. Phillips |
| 250 ## - MENCIÓN DE EDICIÓN |
|---|
| Mención de edición |
2nd edition 2006 |
| 264 #1 - PRODUCCIÓN, PUBLICACIÓN, DISTRIBUCIÓN, FABRICACIÓN Y COPYRIGHT |
|---|
| Producción, publicación, distribución, fabricación y copyright |
Totowa, NJ |
| Nombre del de productor, editor, distribuidor, fabricante |
Humana Press |
| Fecha de producción, publicación, distribución, fabricación o copyright |
2006 |
| 300 ## - DESCRIPCIÓN FÍSICA |
|---|
| Extensión |
1 recurso en línea (XIV, 364 páginas) |
| Otras características físicas |
47 ilustraciones |
| 336 ## - TIPO DE CONTENIDO |
|---|
| Término de tipo de contenido |
texto |
| Código de tipo de contenido |
txt |
| Fuente |
rdacontent |
| 337 ## - TIPO DE MEDIO |
|---|
| Nombre/término del tipo de medio |
electrónico |
| Código del tipo de medio |
c |
| Fuente |
rdamedia |
| 338 ## - TIPO DE SOPORTE |
|---|
| Nombre/término del tipo de soporte |
recurso electrónico |
| Código del tipo de soporte |
cr |
| Fuente |
rdacarrier |
| 347 ## - CARACTERÍSTICAS DEL ARCHIVO DIGITAL |
|---|
| Tipo de archivo |
archivo de texto |
| Formato de codificación |
PDF |
| 490 0# - MENCIÓN DE SERIE |
|---|
| Mención de serie |
Methods in Molecular Biology |
| Número Internacional Normalizado para Publicaciones Seriadas |
1940-6029 |
| Designación de volumen o secuencia |
320 |
| 505 0# - NOTA DE CONTENIDO CON FORMATO |
|---|
| Nota de contenido con formato |
Cytochrome P450 Nomenclature, 2004 -- Spectral Analyses of Cytochromes P450 -- Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli -- Purification of Cytochromes P450 -- Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System -- Cytochrome P450 Reconstitution Systems -- Catalytic Assays for Human Cytochrome P450 -- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate -- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation -- Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes -- High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6?-Hydroxylation -- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography -- CYP2C19-Mediated (S)-Mephenytoin 4?-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection -- CYP2D6-Dependent Bufuralol 1?-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection -- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation -- Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation -- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection -- An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7?-Hydroxylation -- Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families -- Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity -- Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases -- Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes -- The Human Cytochrome P450 Allele Nomenclature Committee Web Site -- Genotyping for Cytochrome P450 Polymorphisms -- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing -- Isolation of Rat Hepatocytes -- Rat Hepatocyte Cultures -- Rat Hepatocyte Cultures -- Hepatocytes in Suspension -- Isolation of Rat Bone Marrow Stem Cells -- Transfection of Primary Cultures of Rat Hepatocytes -- Human Hepatocyte Culture -- Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization -- Deletion of Genes From the Mouse Genome Using Cre/loxP Technology -- Characterization of Targeted Mouse Embryonic Stem Cell Chromosomes -- Microinjection of Targeted Embryonic Stem Cells and Establishment of Knockout Mouse Lines for Fmo Genes -- Use of Reporter Genes to Measure Xenobiotic-Mediated Activation of CYP Gene Transcription. |
| 520 ## - SUMARIO, ETC. |
|---|
| Sumario, etc. |
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Also described are techniques for the generation of mice with targeted gene disruptions. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source. Each chapter is written by researchers who have been involved in the development and application of the particular technique to P450s or FMOs. The protocols are presented in a step-by-step manner, with extensive cross-references to notes that highlight critical steps, potential problems, and alternative methods so that the researcher can understand the basis of the method and perform it successfully. Cutting-edge and highly practical, Cytochrome P450, Second Edition provides both novices and experienced researchers across many fields all the tools needed today to elucidate the crucial biological role played by cytochrome P450 in the metabolism of therapeutic drugs, chemical carcinogens, and environmental pollutants. |
| 988 ## - NOTA LOCAL 598 |
|---|
| Nota local 598 (boletines) |
Springer_Protocols_2006 |
| 650 #7 - PUNTO DE ACCESO ADICIONAL DE MATERIA--TÉRMINO DE MATERIA |
|---|
| Fuente del encabezamiento o término |
embne |
| 9 (RLIN) |
666050 |
| Término de materia o nombre geográfico como elemento de entrada |
Metaloenzimas |
| Subdivisión de forma |
Manuales de laboratorio |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781617375729 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
|---|
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781588294418 |
| 856 40 - LOCALIZACIÓN Y ACCESO ELECTRÓNICOS |
|---|
| Identificador Uniforme del Recurso |
https://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599982 |
| Nota pública |
Acceso a este recurso digital (usuarios Universidad Europea de Madrid) |
| 942 ## - ELEMENTOS DE PUNTO DE ACCESO ADICIONAL (KOHA) |
|---|
| Fuente del sistema de clasificación o colocación |
Library of Congress Classification |
| Tipo de ítem Koha |
LIBRO-E NO PRÉSTAMO |
| 998 ## - DATOS ESTADÍSTICOS |
|---|
| Fecha de catalogación |
09/2023 |
| Tipo de materia |
E-book |
| Catalogador |
Sara Morillo |
| Catalogado |
Sí |