MARC details
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| campo de control de longitud fija |
05851nam a22003855i 4500 |
| 001 - NÚMERO DE CONTROL |
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391950 |
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ES-MaUEC |
| 005 - FECHA Y HORA DE LA ÚLTIMA TRANSACCIÓN |
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20230622111204.0 |
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230622s2005 xxu| o |||| 0|eng d |
| 020 ## - NÚMERO INTERNACIONAL ESTÁNDAR DEL LIBRO |
| Número Internacional Estándar del Libro |
9781592599226 |
| 024 7# - IDENTIFICADOR DE OTROS ESTÁNDARES |
| Número estándar o código |
10.1385/1592599222 |
| Fuente del número o código |
doi |
| 040 ## - FUENTE DE LA CATALOGACIÓN |
| Centro catalogador/agencia de origen |
ES-MaUEC |
| Lengua de catalogación |
spa |
| Centro/agencia transcriptor |
ES-MaUEC |
| Centro/agencia modificador |
ES-MaUEC |
| 050 #4 - SIGNATURA TOPOGRÁFICA DE LA BIBLIOTECA DEL CONGRESO |
| Número de clasificación |
RS431 .P75 |
| Número de documento/Ítem |
2005 EB |
| 245 00 - MENCIÓN DE TÍTULO |
| Título |
Therapeutic Proteins : |
| Resto del título |
Methods and Protocols |
| Mención de responsabilidad, etc. |
edited by C. Mark Smales, David C. James |
| 250 ## - MENCIÓN DE EDICIÓN |
| Mención de edición |
1st edition 2005 |
| 264 #1 - PRODUCCIÓN, PUBLICACIÓN, DISTRIBUCIÓN, FABRICACIÓN Y COPYRIGHT |
| Producción, publicación, distribución, fabricación y copyright |
Totowa, NJ |
| Nombre del de productor, editor, distribuidor, fabricante |
Humana Press |
| Fecha de producción, publicación, distribución, fabricación o copyright |
2005 |
| 300 ## - DESCRIPCIÓN FÍSICA |
| Extensión |
1 recurso en línea (XIV, 482 páginas) |
| 336 ## - TIPO DE CONTENIDO |
| Término de tipo de contenido |
texto |
| Código de tipo de contenido |
txt |
| Fuente |
rdacontent |
| 337 ## - TIPO DE MEDIO |
| Nombre/término del tipo de medio |
electrónico |
| Código del tipo de medio |
c |
| Fuente |
rdamedia |
| 338 ## - TIPO DE SOPORTE |
| Nombre/término del tipo de soporte |
recurso electrónico |
| Código del tipo de soporte |
cr |
| Fuente |
rdacarrier |
| 347 ## - CARACTERÍSTICAS DEL ARCHIVO DIGITAL |
| Tipo de archivo |
archivo de texto |
| Formato de codificación |
PDF |
| 490 0# - MENCIÓN DE SERIE |
| Mención de serie |
Methods in Molecular Biology |
| Número Internacional Normalizado para Publicaciones Seriadas |
1940-6029 |
| Designación de volumen o secuencia |
308 |
| 505 0# - NOTA DE CONTENIDO CON FORMATO |
| Nota de contenido con formato |
Biopharmaceutical Proteins -- Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli -- Secretory Production of Therapeutic Proteins in Escherichia coli -- Expression of Recombinant LTB Protein in Marine vibrio VSP60 -- Heterologous Gene Expression in Yeast -- Pharmaceutical Proteins From Methylotrophic Yeasts -- Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems -- Large-Scale Transient Expression of Therapeutic Proteins in Mammalian Cells -- Site-Specific Integration for High-Level Protein Production in Mammalian Cells -- Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture -- Transgene Control Engineering in Mammalian Cells -- Fusion to Albumin as a Means to Slow the Clearance of Small Therapeutic Proteins Using the Pichia pastoris Expression System -- High-Throughput Recovery of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli -- Isolation, Solubilization, Refolding, and Chromatographic Purification of Human Growth Hormone from Inclusion Bodies of Escherichia coli Cells -- Large-Scale Preparation of Factor VIIa from Human Plasma -- Purification of Clinical-Grade Monoclonal Antibodies by Chromatographic Methods -- Virus Elimination and Validation -- Virus Removal by Nanofiltration -- Determining Residual Host Cell Antigen Levels in Purified Recombinant Proteins by Slot Blot and Scanning Laser Densitometry -- Principles of Biopharmaceutical Protein Formulation -- Solid-State Protein Formulation -- Stabilization of Therapeutic Proteins by Chemical and Physical Methods -- Extraction and Characterization of Vaccine Antigens from Water-in-Oil Adjuvant Formulations -- Probing Reversible Self-Association of Therapeutic Proteins by Sedimentation Velocity in the Analytical Ultracentrifuge -- Biological Characterization of Pegylated Interferons -- Characterization of Interferon ?2B Pegylated via Carboxyalkylation -- Quantifying Recombinant Proteins and Their Degradation Products Using SDS-PAGE and Scanning Laser Densitometry -- Extraction and Denaturing Gel Electrophoretic Methodology for the Analysis of Yeast Proteins -- Characterization of Therapeutic Proteins by Membrane and In-Gel Tryptic Digestion -- Oligosaccharide Release and MALDI-TOF MS Analysis of N-Linked Carbohydrate Structures from Glycoproteins -- Capillary Electrophoresis of Carbohydrates Derivatized With Fluorophoric Compounds -- High-Throughput LC/MS Methodology for ?(1?3)Gal Determination of Recombinant Monoclonal Antibodies -- Carbohydrate Structural Characterization of Fas Ligand Inhibitory Protein -- Top-Down Characterization of Protein Pharmaceuticals by Liquid Chromatography/Mass Spectrometry -- Sample Preparation Procedures for High-Resolution Nuclear Magnetic Resonance Studies of Aqueous and Stabilized Solutions of Therapeutic Peptides. |
| 520 ## - SUMARIO, ETC. |
| Sumario, etc. |
With the completion of the human genome, the number of protein-based drug candidates has increased dramatically, along with the need to find methods for producing these proteins on a scale large enough to meet demand without compromising the authenticity of the final product. In Therapeutic Proteins: Methods and Protocols, world-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Therapeutic Proteins: Methods and Protocols offers both novice and experienced researchers the latest innovative, readily reproducible techniques, as well as helpful commentaries on the development and production of therapeutic proteins, ranging from their expression to actual formulation. |
| 988 ## - NOTA LOCAL 598 |
| Nota local 598 (boletines) |
Springer_Protocols_2005 |
| 650 #7 - PUNTO DE ACCESO ADICIONAL DE MATERIA--TÉRMINO DE MATERIA |
| Fuente del encabezamiento o término |
embne |
| 9 (RLIN) |
139861 |
| Término de materia o nombre geográfico como elemento de entrada |
Proteínas |
| Subdivisión general |
Uso terapéutico |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781588293909 |
| 776 08 - ENTRADA/ENLACE A UN FORMATO FÍSICO ADICIONAL |
| Información de relación/Frase instructiva de referencia |
Printed edition: |
| Número Internacional Estándar del Libro |
9781627038485 |
| 856 40 - LOCALIZACIÓN Y ACCESO ELECTRÓNICOS |
| Identificador Uniforme del Recurso |
https://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592599222 |
| Nota pública |
Acceso a este recurso digital (usuarios Universidad Europea de Madrid) |
| 942 ## - ELEMENTOS DE PUNTO DE ACCESO ADICIONAL (KOHA) |
| Fuente del sistema de clasificación o colocación |
Library of Congress Classification |
| Tipo de ítem Koha |
LIBRO-E NO PRÉSTAMO |
| 998 ## - DATOS ESTADÍSTICOS |
| Fecha de catalogación |
06/2023 |
| Tipo de materia |
E-book |
| Catalogador |
Irene González |
| Catalogado |
Sí |