Protein Dynamics : Methods and Protocols

Protein Dynamics : Methods and Protocols edited by Dennis R. Livesay - 1st edition 2014 - 1 recurso en línea (XIV, 285 páginas) 82 ilustraciones, 56 ilustraciones a color - Methods in Molecular Biology 1084 1940-6029 .

Monitoring Side-Chain Dynamics of Proteins Using 2H Relaxation -- CPMG Relaxation Dispersion -- Confocal Single-Molecule FRET for Protein Conformational Dynamics -- Protein Structural Dynamics Revealed by Site-directed Spin Labeling and Multifrequency EPR -- Probing Backbone Dynamics With Hydrogen/Deuterium Exchange Mass Spectrometry -- Carbon-Deuterium Bonds as Non-perturbative Infrared Probes of Protein Dynamics, Electrostatics, Heterogeneity, and Folding -- Balancing Bond, Nonbond and Gō-like Terms in Coarse Grain Simulations of Conformational Dynamics -- Tutorial on Building Markov State Models with MSMBuilder and Coarse-graining them with BACE -- Analysis of Protein Conformational Transitions Using Elastic Network Model -- Geometric Simulation of Flexible Motion in Proteins -- Principal Component Analysis: A Method for Determining the Essential Dynamics of Proteins -- A Case Study Comparing Quantitative Stability/Flexibility Relationships Across Five Metallo-β-Lactamases Highlighting Differences within NDM-1 -- Towards Comprehensive Analysis of Protein Family Quantitative Stability/Flexibility Relationships using Homology Models -- Using the COREX/BEST Server to Model the Native State Ensemble -- Morphing Methods to Visualize Coarse-grained Protein Dynamics.

In Protein Dynamics: Methods and Protocols, expert researchers in the field detail both experimental and computational methods to interrogate molecular level fluctuations. Chapters detail best-practice recipes covering both experimental and computational techniques, reflecting modern protein research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Protein Dynamics: Methods and Protocols describes the most common and powerful methods used to characterize protein dynamics.  .

9781627036580

10.1007/978-1-62703-658-0 doi


Proteínas--Análisis

QP551 / 2014 EB