High-Throughput Screening Assays in Toxicology
High-Throughput Screening Assays in Toxicology
edited by Hao Zhu, Menghang Xia
- 1st edition 2016
- 1 recurso en línea (XII, 175 páginas) 50 ilustraciones, 32 ilustraciones a color
- Methods in Molecular Biology 1473 1940-6029 .
Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay -- Mitochondrial Membrane Potential Assay -- Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway -- Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators -- Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity -- Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway -- Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells -- Determination of Histone H2AX Phosphorylation in DT40 Cells -- High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells -- Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish -- Fast Functional Germline and Epigenetic Assays in the Nematode
This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.
9781493963461
10.1007/978-1-4939-6346-1 doi
Ensayos de toxicidad
RA1199 / 2016 EB
Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay -- Mitochondrial Membrane Potential Assay -- Using β-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway -- Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators -- Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity -- Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway -- Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells -- Determination of Histone H2AX Phosphorylation in DT40 Cells -- High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells -- Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish -- Fast Functional Germline and Epigenetic Assays in the Nematode
This book focuses on recently developed High Throughput Screening (HTS) assay protocols, many involved in the ToxCast and/or Tox21 initiatives, and the relevant HTS data analysis techniques. Divided into three sections, in vitro assays, in vivo assays, and computational techniques to analyze HTS data are all examined. Written for the highly successful Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High Throughput Screening Assays in Toxicology serves as a valuable reference resource for translating new HTS techniques into standardized chemical toxicity assessment tools in order to advance modern toxicology research to a new era where HTS techniques can partially replace the prevailing animal models.
9781493963461
10.1007/978-1-4939-6346-1 doi
Ensayos de toxicidad
RA1199 / 2016 EB