Site-Specific Protein Labeling : Methods and Protocols
Site-Specific Protein Labeling : Methods and Protocols
edited by Arnaud Gautier, Marlon J. Hinner
- 1st edition 2015
- 1 recurso en línea (XII, 267 páginas) 72 ilustraciones, 34 ilustraciones a color
- Methods in Molecular Biology 1266 1940-6029 .
How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag -- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery -- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins -- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes -- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues -- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels -- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells -- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins -- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing -- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins -- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling -- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist -- BONCAT - Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes -- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins -- Labeling Proteins By Affinity-Guided DMAP Chemistry -- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters present a comprehensive overview on the most relevant and established labeling methodologies, and help researchers to choose the most appropriate labeling method for their biological question. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols is designed to help scientists from all fields to get a better understanding of labeling techniques.
9781493922727
10.1007/978-1-4939-2272-7 doi
Proteínas--Análisis
QP551 / 2015 EB
How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag -- Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery -- Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins -- Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes -- 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues -- Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels -- Fluorescent Labeling Of SNAP-Tagged Proteins In Cells -- HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins -- Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing -- Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins -- Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling -- Site-Specific Biotinylation Of Purified Proteins Using BirA.- Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist -- BONCAT - Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes -- Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins -- Labeling Proteins By Affinity-Guided DMAP Chemistry -- Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo.
This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters present a comprehensive overview on the most relevant and established labeling methodologies, and help researchers to choose the most appropriate labeling method for their biological question. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols is designed to help scientists from all fields to get a better understanding of labeling techniques.
9781493922727
10.1007/978-1-4939-2272-7 doi
Proteínas--Análisis
QP551 / 2015 EB