Pyrosequencing Protocols
Pyrosequencing Protocols
edited by Sharon Marsh
- 1st edition 2007
- 1 recurso en línea (XII, 196 páginas)
- Methods in Molecular Biology 373 1940-6029 .
The History of Pyrosequencing® -- Pyrosequencing® Applications -- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing® -- Pyrosequencing® -- Universal Primer Applications for Pyrosequencing® -- Allele Quantification and DNA Pooling Methods -- Multiplex Pyrosequencing® for DNA Variation Analysis -- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology -- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria -- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci -- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands -- Gene Copy Number Detection in Animal Studies -- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing® -- Pyrosequencing® Genotype Storage Techniques.
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
9781597453776
10.1385/1597453773 doi
Nucleótidos
ADN--Análisis
QP624 / 2007 EB
The History of Pyrosequencing® -- Pyrosequencing® Applications -- Web-Based Primer Design Software for Genome-Scale Genotyping by Pyrosequencing® -- Pyrosequencing® -- Universal Primer Applications for Pyrosequencing® -- Allele Quantification and DNA Pooling Methods -- Multiplex Pyrosequencing® for DNA Variation Analysis -- Analysis of Gene-Specific DNA Methylation Patterns by Pyrosequencing® Technology -- Detecting Mutations That Confer Oxazolidinone Resistance in Gram-Positive Bacteria -- Pyrosequencing®-Based Strategies for Improved Allele Typing of Human Leukocyte Antigen Loci -- Pyrosequencing® of Phage Display Libraries for the Identification of Cell-Specific Targeting Ligands -- Gene Copy Number Detection in Animal Studies -- Detection of Allelic Imbalance in Gene Expression Using Pyrosequencing® -- Pyrosequencing® Genotype Storage Techniques.
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
9781597453776
10.1385/1597453773 doi
Nucleótidos
ADN--Análisis
QP624 / 2007 EB