Antibody Engineering Protocols
Antibody Engineering Protocols
edited by Sudhir Paul
- 1st edition 1995
- 1 recurso en línea (XII, 450 páginas)
- Methods in Molecular Biology 51 1940-6029 .
SEQHUNT -- Molecular Modeling of Antibody-Combining Sites -- Structure and Properties of Human Immunoglobulin Light-Chain Dimers -- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins -- Detection of Human Variable Gene Family Expression at the Single-Cell Level -- Purification of Reduced and Alkylated Antibody Subunits -- Murine Monoclonal Antibody Development -- Comparative Properties of Polyclonal and Monoclonal Antibodies -- Evaluation of Antibody Clonality -- Affinity Immunoblotting -- Epitope and Idiotope Mapping Using Monoclonal Antibodies -- Anti-Idiotypic Antibodies That Mimic Opioids -- Catalytic Antibodies -- Preparation and Assay of Acetylcholinesterase Antibody -- DNA Hydrolysis by Antibodies -- Screening Strategies for Catalytic Antitransition-State Analog Antibodies -- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells -- Single-Chain Anti-DNA Fv -- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments -- Cloning and Bacterial Expression of an Esterolytic sFv -- Site-Directed Mutagenesis of Antibody-Variable Regions -- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation -- Chaperonins in Phage Display of Antibody Fragments -- Phage-Display Libraries of Murine and Human Antibody Fab Fragments -- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library -- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography -- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening -- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies -- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies -- Radiolabeling of Antibodies for Therapy and Diagnosis.
9781592595389
10.1385/0896032752 doi
Inmunoglobulinas
TP248.65 .I49 / 1995 EB
SEQHUNT -- Molecular Modeling of Antibody-Combining Sites -- Structure and Properties of Human Immunoglobulin Light-Chain Dimers -- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins -- Detection of Human Variable Gene Family Expression at the Single-Cell Level -- Purification of Reduced and Alkylated Antibody Subunits -- Murine Monoclonal Antibody Development -- Comparative Properties of Polyclonal and Monoclonal Antibodies -- Evaluation of Antibody Clonality -- Affinity Immunoblotting -- Epitope and Idiotope Mapping Using Monoclonal Antibodies -- Anti-Idiotypic Antibodies That Mimic Opioids -- Catalytic Antibodies -- Preparation and Assay of Acetylcholinesterase Antibody -- DNA Hydrolysis by Antibodies -- Screening Strategies for Catalytic Antitransition-State Analog Antibodies -- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells -- Single-Chain Anti-DNA Fv -- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments -- Cloning and Bacterial Expression of an Esterolytic sFv -- Site-Directed Mutagenesis of Antibody-Variable Regions -- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation -- Chaperonins in Phage Display of Antibody Fragments -- Phage-Display Libraries of Murine and Human Antibody Fab Fragments -- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library -- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography -- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening -- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies -- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies -- Radiolabeling of Antibodies for Therapy and Diagnosis.
9781592595389
10.1385/0896032752 doi
Inmunoglobulinas
TP248.65 .I49 / 1995 EB