Renal Cancer : Methods and Protocols
Renal Cancer : Methods and Protocols
edited by Jack H. Mydlo
- 1st edition 2001
- 1 recurso en línea (XII, 403 páginas) 55 ilustraciones
- Methods in Molecular Medicine 53 1940-6037 .
Renal Cell Carcinoma -- Pathology of Kidney Tumors -- In Vitro Experimental Techniques -- Telomerase Assay in Renal Cancer -- Comparative Genomic Hybridization -- Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry -- Detection of Apoptosis in Renal Cell Carcinoma -- Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes -- Polymerase Chain Reaction Detection of DNA Sequence Deletions -- Matrix Metalloproteinases and Their Inhibitors -- Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGF?, and EGFR -- Laser-Capture Microdissection -- Expression of c-met and WT-1 -- Molecular Analysis of the von Hippel-Lindau Disease Gene -- Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma -- In Vivo Experimental Techniques -- Orthotopic Model of Renal Cell Carcinoma -- Murine Animal Model -- Angiogenesis Assays -- Antiangiogenic Approaches to Renal Cell Carcinoma -- Clinical Trials and Experiments -- Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma -- Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination -- Monoclonal Antibodies -- Treatment Strategies in Metastatic Renal Cell Carcinoma -- 23 Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma -- Transforming Growth Factor-?1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma -- Static and Flow Cytometry.
Because renal cancer can be managed successfully only when localized, there is a great need to develop new treatments for patients with advanced or metastatic disease. In Renal Cancer: Methods and Protocols, Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological therapies that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. In vivo assays are provided for analyzing angiogenesis, anti-angiogenesis, and general renal tumor biology as a prelude to human clinical trials. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.
9781592591442
10.1385/1592591442 doi
Riñones--Cáncer--Manuales de laboratorio
RC280 .K5 / 2001 EB
Renal Cell Carcinoma -- Pathology of Kidney Tumors -- In Vitro Experimental Techniques -- Telomerase Assay in Renal Cancer -- Comparative Genomic Hybridization -- Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry -- Detection of Apoptosis in Renal Cell Carcinoma -- Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes -- Polymerase Chain Reaction Detection of DNA Sequence Deletions -- Matrix Metalloproteinases and Their Inhibitors -- Purification of Growth Factor mRNA in Renal Tissues:bFGF-2, FGF-2, TGF?, and EGFR -- Laser-Capture Microdissection -- Expression of c-met and WT-1 -- Molecular Analysis of the von Hippel-Lindau Disease Gene -- Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma -- In Vivo Experimental Techniques -- Orthotopic Model of Renal Cell Carcinoma -- Murine Animal Model -- Angiogenesis Assays -- Antiangiogenic Approaches to Renal Cell Carcinoma -- Clinical Trials and Experiments -- Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma -- Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination -- Monoclonal Antibodies -- Treatment Strategies in Metastatic Renal Cell Carcinoma -- 23 Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma -- Transforming Growth Factor-?1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma -- Static and Flow Cytometry.
Because renal cancer can be managed successfully only when localized, there is a great need to develop new treatments for patients with advanced or metastatic disease. In Renal Cancer: Methods and Protocols, Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological therapies that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. In vivo assays are provided for analyzing angiogenesis, anti-angiogenesis, and general renal tumor biology as a prelude to human clinical trials. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.
9781592591442
10.1385/1592591442 doi
Riñones--Cáncer--Manuales de laboratorio
RC280 .K5 / 2001 EB