Cardiac Cell and Gene Transfer
Cardiac Cell and Gene Transfer
edited by Joseph M. Metzger
- 1st edition 2003
- 1 recurso en línea (XI, 253 páginas)
- Methods in Molecular Biology 219 1940-6029 .
Vectors for Cardiac Gene Transfer -- Adenoviral Vectors -- Gutted Adenoviral Vectors for Gene Transfer to Muscle -- Dual Vector Expansion of the Recombinant AAV Packaging Capacity -- Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes -- Cell Transfer to Myocardium -- Cell Therapy in the Heart -- Cardiac Cell Transplantation -- Cell Grafting for Cardiac Repair -- Vectors for Cardiac Gene Transfer -- Suppressor tRNAs -- Antisense Oligonucleotides -- Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants -- Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes -- Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes -- In Vivo Cardiac Gene Transfer -- Embryonic and Neonatal Cardiac Gene Transfer In Vivo -- Efficient Viral Gene Transfer to Rodent Hearts In Vivo -- Direct Gene Transfer to the Adult Rodent Myocardium In Vivo -- Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures -- Coronary Perfusion Cocktails for In Vivo Gene Transfer -- Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of ?-Adrenergic Receptor Signaling Components -- Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo.
Cellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many novel techniques and strategies for the cellular and genetic modification of heart function. These highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.
9781592593507
10.1385/159259350X doi
Corazón--Enfermedades--Manuales de laboratorio
Terapia genética--Manuales de laboratorio
RC684 .G44 / 2003 EB
Vectors for Cardiac Gene Transfer -- Adenoviral Vectors -- Gutted Adenoviral Vectors for Gene Transfer to Muscle -- Dual Vector Expansion of the Recombinant AAV Packaging Capacity -- Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes -- Cell Transfer to Myocardium -- Cell Therapy in the Heart -- Cardiac Cell Transplantation -- Cell Grafting for Cardiac Repair -- Vectors for Cardiac Gene Transfer -- Suppressor tRNAs -- Antisense Oligonucleotides -- Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants -- Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes -- Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes -- In Vivo Cardiac Gene Transfer -- Embryonic and Neonatal Cardiac Gene Transfer In Vivo -- Efficient Viral Gene Transfer to Rodent Hearts In Vivo -- Direct Gene Transfer to the Adult Rodent Myocardium In Vivo -- Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures -- Coronary Perfusion Cocktails for In Vivo Gene Transfer -- Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of ?-Adrenergic Receptor Signaling Components -- Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo.
Cellular and gene-based experimental techniques promise powerful new therapeutic agents for the remediation of both acquired and inherited cardiovascular disease. In Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications, Joseph M. Metzger and a panel of leading scientific experts detail many novel techniques and strategies for the cellular and genetic modification of heart function. These highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today's unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.
9781592593507
10.1385/159259350X doi
Corazón--Enfermedades--Manuales de laboratorio
Terapia genética--Manuales de laboratorio
RC684 .G44 / 2003 EB