Normal view MARC view ISBD view

Protein Purification Protocols edited by Shawn Doonan - 1st edition 1996 - 1 recurso en línea (X, 406 páginas) - Methods in Molecular Biology 59 1940-6029 .

General Strategies -- Preparation of Extracts from Animal Tissues -- Protein Extraction from Plant Tissues -- Extraction of Recombinant Protein from Bacteria -- Protein Extraction from Fungi -- Subcellular Fractionation of Animal Tissues -- Subcellular Fractionation of Plant Tissues -- The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds -- Avoidance of Proteolysis in Extracts -- Concentration of Extracts -- Making and Changing Buffers -- Purification and Concentration by Ultrafiltration -- Bulk Purification by Fractional Precipitation -- Ion-Exchange Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Dye-Ligand Affinity Chromatography -- Lectin Affinity Chromatography -- Immunoaffinity Chromatography -- Immobilized Metal Ion Affinity Chromatography -- Chromatography on Hydroxyapatite -- Affinity Precipitation Methods -- Isoelectric Focusing -- Chromatofocusing -- Size-Exclusion Chromatography -- Fast Protein Liquid Chromatography (FPLC) Methods -- Reversed-Phase Chromatography of Proteins -- Extraction of Membrane Proteins -- Removal of Detergent from Protein Fractions -- Purification of Membrane Proteins -- Lyophilization of Proteins -- Storage of Pure Proteins -- Electroelution of Proteins from Polyacrylamide Gels -- Electroblotting of Proteins from Polyacrylamide Gels -- High-Performance Electrophoresis Chromatography -- Practical Column Chromatography.

Hans Neurath has written that this is the second golden era of enzymology

ISBN: 9781592595457

Standard No.: 10.1385/0896033368 doi

Subjects--Topical Terms:
Proteínas--Manuales de laboratorio

LC Class. No.: QP551 / 1996 EB